Which of the following is the LEAST likely ER signal sequence? All sequences are written with their N-terminus on the left. O Met-Lys-Leu-Ser-Leu-Val-Ala-Ala-lle-Leu-Leu-Leu-Leu-Ser-Ala-Ala-Arg-Ala Met-Glu-Met-Phe-Gln-Gly-Leu-Leu-Leu-Leu-Leu-Leu-Leu-Leu-Ser-Met-Gly-Gly-Thr- Trp-Ala O Met-Lys-Ala-Lys-Leu-Leu-Val-Leu-Leu-Tyr-Ala-Phe-Val-Ala-Gly-Asn Met-Leu-Ser-Lys-Arg-Gln-Ser-Lys-Arg-Arg-Lys-Lys-Pro-Ala-Thr-Arg-Thr-Lys-Ser- Ser-Arg-Tyr-Leu

The figure  from Moore (2020) shows a G protein-coupled receptor in a membrane. Note that "out" means outside the cell and "in" means facing the cytoplasm. The three amino acids "DRY" in loop I2 are required  for protein targeting. What is the name of this kind  of sequence, and what is its function? What protein targeting sequence is no longer included in this diagram? Why? The sequence "QXXNK" (where X is any amino acid) found in loop I3 has been identified based on its enzymatic activity. What is this activity? What particular domain would you expect to find in either Loop E1, E2, or E3?  The gene that codes for this protein is a member of  a family of genes that has arisen over evolutionary time. If you compared several of the genes in this family, would you expect their sequences to be most homologous (similar) in the region you describe in #3 above or #4 above? Why?

Why might a protein precipitate when its tag is cleaved?

If you were to remove the ER retrieval signal fromprotein disulfide isomerase (PDI), which is normally a sol-uble resident of the ER lumen, where would you expect themodified PDI to be located?

About how many GTps does it take to synthesize a protein 100 amino acids long?

2010-10194 E / 65029030 + +H OTU Domain What is designated by 'rs'? Please expalin (50 words or less) E3 Igaso Zinc Finger Region

Why are suppressor tRNA mutation not lethal

The sigma-70 promoter is very well-studied. The Sigma-54 promoter, on the other hand, hasn't had quite as much study devoted to it, but a consensus sequence for Sigma-54 promoters has been determined, and it looks like this: C GAAA FICGCACTECT УА C 5-26-25-24 -23 -22 -21 -20 -19 -18 -17 -16-15-14-13-12-11 -10 3 wablogo berkalay.edu (From Franke et. Al, 2011 BMC Genomics) If you were studying a gene with a promoter that exactly matched the consensus sequence, which base would, if mutated, probably make the largest difference in the ability of Sigma-54 to bind to a promoter? O Base position 26 Base position 10 Base position 12 Base position 16

In posttranslational modification, phosphates are attached to which 3 amino acids?  - tyrosine - serine - proline - lysine - threonine

After the GEF causes the GTPase to release its GDP, why is the nucleotide-binding site immediately filled by a GTP molecule rather than a GDP molecule?