which of the following can be used as a disinfectant on a table top and works by denaturing bacteria lipids and membranes? 1-1% tincture of iodine 2-37% Formalin 3-Crystal Violet 4-Hand soap 5-Ethanol
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which of the following can be used as a disinfectant on a table top and works by denaturing bacteria lipids and membranes?
1-1% tincture of iodine
2-37% Formalin
3-Crystal Violet
4-Hand soap
5-Ethanol
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- Below are the results of our disc diffusion study using antiseptics and disinfectants. Use the results to answer the following questions: 1:10 Lab diluted Lab Tincture bleach Disinfectant Isopropanol soap Lysol of iodine BL DIS ISP SO LYS T of I E.coli 40 mm 20 mm 10 mm 5mm 15mm 60 mm Bacillus 50 mm 35 mm 15 mm 8mm 20mm 70 mm cereus chemical (s) best at inhibiting both Gram- negative and Gram-positive bacteria [Choose ] [Choose] chemical least effective at inhibiting both Soap bacteria 1:10 freshly diluted bleach chemical recommended by CDC for decontamination of surfaces contaminate with blod possibly carrying HIV/Hepatitis B or C viruses Tincture of lodineBelow are the results of our disc diffusion study using antiseptics and disinfectants. Use the results to answer the following questions: 1:10 Lab diluted Lab Tincture bleach Disinfectant Isopropanol soap Lysol of iodine BL DIS ISP SO LYS T of I E.coli 40 mm 20 mm 10mm 5mm 15mm 60 mm Bacillus 50 mm 35 mm 15 mm 8mm 20mm 70 mm cereus chemical(s) best at inhibiting both Gram- negative and Gram-positive bacteria [ Choose ] chemical least effective at inhibiting both [ Choose ] bacteriaWhich of the following are true regarding UV disinfection? Mark all that apply. Water must be clear, so light can kill pathogens It is the cheapest method of disinfection The water has to flow at a specific speed past the UV light Only about 85% of pathogens are killed by UV It is very rarely used in water disinfection due to high costs
- List chemical agents that are effective at disinfecting smaller surfaces? Question 15 options: hydrogen perioxide steam Ultraviolet (UV) light Copper and/or silver coated surfaces All of the above Steam and UV light onlyWhen a disinfectant or antiseptic's effectiveness is expressed as a phenol coefficient, a number ____ 1.0 means the chemical agent is more effective than phenol. less than greater than equal to5 1 2 6 Testing Anti-Microbial Agents: Disk-Diffusion. Lab Report Fill out the zones of inhibition table with your data: Agent Tested Cleansing Spray 3 4 Foaming Handwash Hand Sanitizer Lysol spray Detergent Desinfecting zone of inhibition: (mm) E. coli 10mm 15 mm 20mm 0mm 9mm P. aeruginosa 8 mm 35mm 17mm 15mm 0 mm 1mm Hydrogen Peroxide Here, write a few observations about your experimental results. 25mm S. aureus 25mm 20mm 25mm 12mm 8mm 42mm What factors (at least 2) other than the identity of the agent being tested can influence the size of the zone of inhibition? NOT Were any colonies observed within the zone of inhibition? Even if you didn't have any, explain why you might see colonies in the zone of inhibition. Be specific about how that might happen? What follow up questions or conclusions do you have related to your data? (at least 2)
- A lab technician is working with a bacterium in pure culture (in 5 ml of liquid media in a test tube). The bacterium is a mesophile that can infect humans. Which of the following is NOT true (with regards to temperature conditions for this bacterial culture)? Lowering the temperature to -10 deg C for at least an hour will likely kill all the bacteria. Placing the tube at 37 deg C will likely facilitate rapid growth of the bacteria. Raising the temperature to 90 deg C for at least an hour will likely kill all the bacteria. Placing the tube at 4 deg C will likely slow or halt growth of the bacteria.Examine the given sets of table below. Which disinfectant is the MOST recommended? Support and Justify your answer. Table 2. Phenol Coefficient of Test Disinfectant Name of disinfectant Growth after 5 minutes Dettol Phenol Name of disinfectant Z-germicSide Phenol Dilution 1:100 1:125 1:150 1:175 1:200 1:95 1:100 1:105 1:110 1:115 Dilution 1:100 1:125 1:150 1:175 1:200 1:95 1:100 1:105 1:110 1:115 - + + + + * + Growth after 5 mins + + + Growth after 10 minutes + + + + Growth after 10 minutes + + * Growth after 15 minutes + + + + + Growth after 15 mins + P .. + 9. + + +What is the physical appearance (solid, liquid, semi-solid) of following media: agar plate
- Examine the given sets of table below. Which disinfectant is the MOST recommended? Support and Justify your answer. Table 2. Phenol Coefficient of Test Disinfectant Name of disinfectant Growth after 5 minutes Dettol Phenol Name of disinfectant Z-germicSide Phenol Name of disinfectant Jik Phenol KEY:- = No growth, + = Growth Dilution 1:100 1:125 1:150 1:175 1:200 1:95 1:100 1:105 1:110 1:115 Dilution 1:100 1:125 1:150 1:175 1:200 1:95 1:100 1:105 1:110 1:115 Dilution 1:100 1:125 1:150 1:175 1:200 1:95 1:100 1:105 1:110 1:115 + + + + + + + + Growth after 5 mins + + + + + + + Growth after 5 mins + + + + + + Growth after 10 minutes + + + + + Growth after 10 minutes + + + + + Growth after 10 mins + - + + + + + + Growth after 15 minutes + + + + + + Growth after 15 mins - + + + + + Growth after 15 mins + + + + - + + + +Put the following Steps of gram smear in order Create a bacteria Add crystal violet to the bacterial smear. Rinse with water after 60 seconds. Allow the smear to air dry and then heat fix it. Add gram iodine to the smear, let it sit for 60 seconds and rinse water decolorize with 95% ethanolfoe 5-10 seconds and then rise with water Blot dry with bibulous paper Add safranin to the smear and let sit for 60 seconds, then rinse with water Observe using oil immersionOne of the following matches indicatIng early microbial spoilage of pharmaceutical products is incorrect Select one: Microbial pigments / Color changes Amines / Unpleasant fishy smelling and tasting Fatty acids / Sour taste Depolymerization of suspending agents / Production of slimy and viscous masses