When polybrene is injected into a silica capillary, positively charged ammonium groups adhere to the —SiO2 groups, converting the net charge on the wall from negative to positive. A capillary is set up with the injector end negative and the detector end positive. In what order will cations, anions, and neutral molecules be eluted?
When polybrene is injected into a silica capillary, positively charged ammonium groups adhere to the —SiO2 groups, converting the net charge on the wall from negative to positive. A capillary is set up with the injector end negative and the detector end positive. In what order will cations, anions, and neutral molecules be eluted?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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When polybrene is injected into a silica capillary, positively charged ammonium groups adhere to the —SiO2 groups, converting the net charge on the wall from negative to positive. A capillary is set up with the injector end negative and the detector end positive. In what order will cations, anions, and neutral molecules be eluted?
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