When Laybourne and Kadonaga studied the effects of histone proteins on eukaryotic transcription using an in vitro transcription assay explain why: a) they used two different DNA templates that contained different promoter structures. b) when they included both activator protein and histones, they always added the histone protein before adding the activator to the transcription assay mixture. (Ctri) -

How do I draw the sequence and explain the process for each step? Draw the sequence of molecular events that occurs to induce STAT transcription factor localization to the nucleus. To complete this, you will draw the first step that occurs, then draw a new figure with the second step that occurs, then draw a new figure with the third step that occurs, and so on until you have completed all of the steps. On the drawing, briefly label each molecular event (each drawing). For this brief label, explain what is happening during each step.

Explain why inserting 5 bp of DNA at the –50 position of a eukaryotic gene decreases the rate of RNA polymerase II transcription initiation to a greater extent than inserting 10 bp at the same site.

a.) Draw a eukaryotic gene with four exons (include the location of the promoter, enhancer, start and stop codon, poly-A signal sequence, 5' UTR, and 3'UTR.). b.) Draw the primary transcript. c.) Draw all of the possible fully mature mRNAs that can be produced from this locus.