What would be the independent variables for the spinach leaf serial dilution experiment

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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What would be the independent variables for the spinach leaf serial dilution experiment 

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The spinach leaf serial dilution experiment is a method, used to measure the rate of photosynthesis when spinach leaves are submerged in a concentration of CO2 and water. 

In this experiment, 10 uniform shaped spinach leaf discs were dipped in a solution of Sodium Bicarbonate and in water and it was observed that spinach leaves dipped in Sodium Bicarbonate solution float up faster, indicating higher rates of Photosynthesis. 

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