What tests would you use to determine if an organism was fermentation positive or negative? Include in your answer the indicator you would use. In addition to carbohydrate, what other major organic material, present in the medium, could be broken down to produce energy and what would be the final color change be  used when it is broken down?. Also indicate  the three possible color changes of the indicator used and the three pH levels at which the medium changes color. Explain the purpose of the Durham or fermentation tube in the test tube and why it is upside down? What gas is produced in fermentation of glucose?  The purpose of the fermentation tests are to determine members of what family from other gram negative organisms?  Name a genera of Gram positive cocci from a genera of Gram positive cocci which is a non-fermenter.

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Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.