Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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what scientistss showed that short pulses of tritiated thymidine, in rapidly growing bacterial cells?
And what was the results in which of the following findings during sucrose density gradient centrifugation?
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- For the study of alanine production by a recombinant strain of E. coli, cultivation was carried out in a benchtop bioreactor with 4.5 L of culture medium, using glucose as a limiting substrate. During the cultivation, there was no lag phase and the cells showed exponential growth for 5 hours. The following table presents the results of the analysis of ammonia and glucose consumption, and alanine accumulation throughout the cultivation. Knowing that 500 mL of a cell suspension at a concentration of 5.0 g/L (inoculum) was added to the 4.5 L of medium in the reactor and that the YX/NH3 previously determined was 7.5, calculate:a) the maximum specific growth rateb) YX/S and YP/S yield factorsc) How long would it take to reach Cx = 30 g/L if the cells continued with the exponential growth profile until the end of the culture (without nutrient deprivation or any type of inhibition)?d) Describe how the mathematical treatment of the data should be done to determine the type of product formation…arrow_forwardWhat is the result of this biochemical test in the gelatin deep? Which enzyme is this testing for? What tool is used to inoculate this medium? Gelatin positive liquid, use needle, protease,arrow_forwardLabel the NMR and tell what each peak representsarrow_forward
- Oops! Your pipettor wasn’t set correctly and you only pipetted 87 µl of a 10-7 dilution when plating serial dilutions. But, you noted this error in your lab notebook so you could correctly perform the calculation. When this plate was grown, you counted 147 colonies on the plate. What was the concentration of bacteria in the original, undiluted suspension?arrow_forwardWhat is the expected yield for plasma membranes isolated by sucrose gradient centrifugation from mouse tissues?arrow_forwardDescribe unique characteristics of agarose used in electrophoresis.arrow_forward
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