What media do we use to visualize and differentiate hemolytic bacteria? List the different types of hemolysis and describe what they would look like on laboratory media.

What is the purpose of fixing a smear? Mark all that apply: 1. To attach the bacteria to the slide  2. To cause the cells to shrink and become distorted 3. To kill the bacteria so they aren't harmed by the staining method 4. To break down the cell wall in order to make the cells accept stain 5. To kill the bacteria to make the slide safer to handle

What test can help you differentiate staphylococci from streptococci? What reagent or media would be use? short answer only

Which of the following plates (dilution factor) would you count/use to determine the original bacterial count? 1 ml Original inoculum Dilutions Plating 1:10 9 ml broth in each tube 1:10 1 ml 1 ml 1 ml 1:100 1 ml 1:100 2 (1:100) 3 (1:1000) 4 (1:10,000) 5 (1:100,000) 1:1000 1 ml 1 ml 1:1000 1:10,000 1 ml 1 ml 1:10,000 1:100,000 1 ml 1:100,000 Calculation: Number of colonies on platex reciprocal of dilution of sample=number of bacteria/ml (For example, if 32 colonies are on a plate of 1/10,000 dilution, then the count is 32 x 10,000 = 320,000/ml in sample.) Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings