What is the role of these organisms in the Winogradsky column and what are the physical indications that they grew inside the column? Cellulose degraders Sulfate reducers Sulfur oxidizers
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What is the role of these organisms in the Winogradsky column and what are the physical indications that they grew inside the column?
- Cellulose degraders
- Sulfate reducers
- Sulfur oxidizers
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- What nutrients do the following media components contain? Peptone Yeast extract Beef extract Potato infusion Agar Why should agar media be completely dissolved before they are dispensed in tubes and plates? What are the bases for pegging the temperature at 1210C for 15-30 minutes during moist heat sterilization and 1800C for two (2) hours using dry heat sterilization? 1.Can you sterilize culture media using dry heat sterilization? Why is that so? You will notice in the videos shown, the cotton plug is not used. What is role of cotton plug in media prep, sterilization and culture of microorganisms? Instead of using cotton plug, plastic screw-cap is used, can you substitute this for the former? Is it technically acceptable in microbiology?Please include sourceWhat type of agar is MacConkey agar?The following ingredient list represents which type of growth media? Beef extract 1.5 g Yeast extract 3.0 g Peptone 6.0 g Glucose 1.0 g Agar 15.0 g water 1000 ml pH 6.6
- Of what practical importance are air borne microorganisms to the laboratory workers? What precautions should be taken to control laboratory contaminants? Why are petri dishes incubated in an inverted position? Of what advantage is the using a solid and a liquid medium?Assume you are the lead industrial microbiologist in a company that produces cholesterol-lowering fungal metabolite such as lovastatin. Your team isolated a novel fungus that can be used for large-scale production of lovastatin. Briefly describe how you would produce 20 L of this product from a potato dextrose agar plate culture using a 20 L fermentor.Mannitol salt agar contains salt to prevent the growth of most microbes and mannitol plus a pH indicator to detect mannitol fermentation. Which component of mannitol salt agar makes it a selective medium?
- MacConkey agar inhibits the growth of Gram-positive bacteria while encouraging the growth of Gram-negative bacteria. It also can determine if some bacteria use lactose. Define what type(s) of media MacConkey agar is, using as many terms in the answer list as are applicable.Answer the following questions: 1. Why is buffering important when preparing a growth media? 2. Explain why bacterial fermentation preserves food from spoilage.In a nutrient broth, where do microorganisms acquire their requirements for mineral elements like Ca, Fe, etc. from? Can gelatin be used in culture media preparation? How is it different from agar? During sterilization, pH of culture media may change. How can this be fixed? How does pH affect the shelf life of unsterilized media?
- Name at least one reason that we might want to use a Winogradsky column rather than a laboratory medium such as nutrient agar or beef heart agar. Describe why metabolism in the presence of oxygen might cause problems for microorganisms.Nitrate reduction to nitrite is indicated by the formation of a pink to red colour after addition of nitrate test reagent. However, if a culture does not produce a colour change several posibilies exist. Explain.What is the EFFECT and RATIONAL of Molten agar mixed with bacterial suspension has temperature higher than 45 oC.