Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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What is the experimental evidence that mitochondria can change conformation during respiration?
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- What are some of the difficulties in extracting respiratory complexes from the inner mitochondrial membrane to investigate their properties?arrow_forwarda) Explain how in oxygenated tissue your cells use your MITOCHONDRIA to produce energy: DESCRIBE the processes occurring in your MITOCHONDRIA (intermediate stage, Krebs, and ETC), Make sure to mention where those processes occur.b) How many ATP per glucose are formed in your mitochondria? Where are they formed?arrow_forwardOf which main compounds is the mitochondrion structure made?arrow_forward
- what is the overall equation for cellular respiration? Name the 4 phases involved in cellular respiration, and when each of the end products of cellular respiration are made. Use all relevant terms.arrow_forwardYeast produces carbon dioxide gas. Therefore, based only on this information, can we tell whether the yeast were using aerobic cellular respiration or Anaerobic (ethanol) fermentation? Yes or No If not, what additional information would we need to know to tell whether the yeast used aerobic respiration or anaerobic (Ethanol) fermentation?arrow_forwardImagine that you develop a procedure to measure the pH of in the matrix and the intermembrane space of a mitochondrion. What pattern of pH would you see? The pH in the intermembrane space will contain more H+ and have a lower pH compared to the matrix. The pH in the intermembrane space will contain more H+ and have a higher pH compared to the matrix. The pH within the matrix will contain more H+ and have a lower pH compared to the intermembrane space. The pH within the matrix will contain more H+ and have a higher pH compared to the intermembrane space.arrow_forward
- If oxygen is not available for the electron transport chain, how is the Krebs cycle affected? What is the alternative pathway that the cells would opt to respire?arrow_forwardWould mitochondrial transmembrane transport of a protein bound for the mitochondria intramembrane space involve TOM, TIM or both?arrow_forwardConsider the malate dehydrogenase reaction, part of tricarboxylic acid cycle, shown below. malate + NAD+ oxaloacetate + NADH + H+ ΔGo ’ = 29.7 kJ/mol. It has been reported that the concentrations of NAD+ and NADH in yeast mitochondria were 20 mM and 0.3 mM, respectively. If we performed similar measurements and also determined that the concentration of malate in yeast mitochondria was 0.5 mM and that of oxaloacetate was 0.1 µM at pH 7.0 at 37˚C, use this information to calculate the free energy of the reaction of yeast in mitochondria.arrow_forward
- Consider the malate dehydrogenase reaction, part of tricarboxylic acid cycle, shown below. malate + NAD+ oxaloacetate + NADH + H+ ΔGo ’ = 29.7 kJ/mol. It has been reported that the concentrations of NAD+ and NADH in yeast mitochondria were 20 mM and 0.3 mM, respectively. If we performed similar measurements and also determined that the concentration of malate in yeast mitochondria was 0.5 mM and that of oxaloacetate was 0.1 µM at pH 7.0 at 37˚C, use this information to calculate the equilibrium constant for the given reaction.arrow_forwardDescribe an experiment that shows if early mouse embryo inner cell mass cells containing mitochondrial defects die through necrosis of apoptosis.arrow_forwardUsing the concepts of endo- and exo-cytosis explain the origin of the mitochondria. Include a drawingarrow_forward
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