Fraction no. Elution Vol. (mL) Abs (λ = 410) Fraction no. Elution Vol. (mL) Abs (λ = 410) 1 (PB pH 6.0) 1 0.0050 11 11 0.0041 2 2 0.0032 12 12 0.0060 3 3 0.0040 13 13 0.0064 4 4 0.0064 14 14 0.0063 5 5 0.0067 15 15 0.1109 6 6 0.0067 16 16 2.8902 7 7 0.0050 17 17 0.8500 8 (PB pH 8.0) 8 0.0049 18 18 0.2019 9 9 0.0052 19 19 0.0378 10 10 0.0050 20 20 0.0073 Use the data above to plot an elution profile, in which the absorbance is on Y-axis, and elution volume (volume = fraction number) is on X-axis. The elution profile must indicate at (1) which point the pH change occurred, (2) the fraction(s) which contain the highest myoglobin content, and (3) the maximum absorbance detected.
Fraction no. Elution Vol. (mL) Abs (λ = 410) Fraction no. Elution Vol. (mL) Abs (λ = 410) 1 (PB pH 6.0) 1 0.0050 11 11 0.0041 2 2 0.0032 12 12 0.0060 3 3 0.0040 13 13 0.0064 4 4 0.0064 14 14 0.0063 5 5 0.0067 15 15 0.1109 6 6 0.0067 16 16 2.8902 7 7 0.0050 17 17 0.8500 8 (PB pH 8.0) 8 0.0049 18 18 0.2019 9 9 0.0052 19 19 0.0378 10 10 0.0050 20 20 0.0073 Use the data above to plot an elution profile, in which the absorbance is on Y-axis, and elution volume (volume = fraction number) is on X-axis. The elution profile must indicate at (1) which point the pH change occurred, (2) the fraction(s) which contain the highest myoglobin content, and (3) the maximum absorbance detected.
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
Related questions
Question
Chemistry
|
Fraction no. |
Elution Vol. (mL) |
Abs (λ = 410) |
|
Fraction no. |
Elution Vol. (mL) |
Abs (λ = 410) |
|
1 |
(PB pH 6.0) 1 |
0.0050 |
|
11 |
11 |
0.0041 |
|
2 |
2 |
0.0032 |
|
12 |
12 |
0.0060 |
|
3 |
3 |
0.0040 |
|
13 |
13 |
0.0064 |
|
4 |
4 |
0.0064 |
|
14 |
14 |
0.0063 |
|
5 |
5 |
0.0067 |
|
15 |
15 |
0.1109 |
|
6 |
6 |
0.0067 |
|
16 |
16 |
2.8902 |
|
7 |
7 |
0.0050 |
|
17 |
17 |
0.8500 |
|
8 |
(PB pH 8.0) 8 |
0.0049 |
|
18 |
18 |
0.2019 |
|
9 |
9 |
0.0052 |
|
19 |
19 |
0.0378 |
|
10 |
10 |
0.0050 |
|
20 |
20 |
0.0073 |
Use the data above to plot an elution profile, in which the absorbance is on Y-axis, and elution volume (volume = fraction number) is on X-axis. The elution profile must indicate at (1) which point the pH change occurred, (2) the fraction(s) which contain the highest myoglobin content, and (3) the maximum absorbance detected.
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
This is a popular solution!
Trending now
This is a popular solution!
Step by step
Solved in 2 steps with 1 images
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.Recommended textbooks for you
Chemistry
Chemistry
ISBN:
9781305957404
Author:
Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:
Cengage Learning
Chemistry
Chemistry
ISBN:
9781259911156
Author:
Raymond Chang Dr., Jason Overby Professor
Publisher:
McGraw-Hill Education
Principles of Instrumental Analysis
Chemistry
ISBN:
9781305577213
Author:
Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:
Cengage Learning
Chemistry
Chemistry
ISBN:
9781305957404
Author:
Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:
Cengage Learning
Chemistry
Chemistry
ISBN:
9781259911156
Author:
Raymond Chang Dr., Jason Overby Professor
Publisher:
McGraw-Hill Education
Principles of Instrumental Analysis
Chemistry
ISBN:
9781305577213
Author:
Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:
Cengage Learning
Organic Chemistry
Chemistry
ISBN:
9780078021558
Author:
Janice Gorzynski Smith Dr.
Publisher:
McGraw-Hill Education
Chemistry: Principles and Reactions
Chemistry
ISBN:
9781305079373
Author:
William L. Masterton, Cecile N. Hurley
Publisher:
Cengage Learning
Elementary Principles of Chemical Processes, Bind…
Chemistry
ISBN:
9781118431221
Author:
Richard M. Felder, Ronald W. Rousseau, Lisa G. Bullard
Publisher:
WILEY