Chemistry
Chemistry
10th Edition
ISBN: 9781305957404
Author: Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher: Cengage Learning
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Chemistry

Fraction no.

Elution Vol. (mL)

Abs                      (λ = 410)

 

Fraction no.

Elution Vol. (mL)

Abs   (λ = 410)

1

(PB pH 6.0) 1

0.0050

 

11

11

0.0041

2

2

0.0032

 

12

12

0.0060

3

3

0.0040

 

13

13

0.0064

4

4

0.0064

 

14

14

0.0063

5

5

0.0067

 

15

15

0.1109

6

6

0.0067

 

16

16

2.8902

7

7

0.0050

 

17

17

0.8500

8

(PB pH 8.0) 8

0.0049

 

18

18

0.2019

9

9

0.0052

 

19

19

0.0378

10

10

0.0050

 

20

20

0.0073

Use the data above to plot an elution profile, in which the absorbance is on Y-axis, and elution volume (volume = fraction number) is on X-axis. The elution profile must indicate at (1) which point the pH change occurred(2) the fraction(s) which contain the highest myoglobin content, and (3) the maximum absorbance detected.

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