What is protein denaturation? Give five agents that can denature proteins  Is it possible for a denatured protein to revert into its native/original form? Please explain

1a) In MALDI-TOF data, when performing peptide mass mapping search,  the number of peptides and the sequence coverage decreases, but protien score increase. Explain b) As the same as part a, the peptide masses and the coverage remain the same, but the protien score increase, explain how this can happen.

The elegance and importance of proper protein folding and assembly cannot be overstated.  Simple electrostatic interactions are fully responsible for long peptide chains becoming giant, globular, functional proteins.  Research and discuss two examples of improper protein folding.  What is the cause of each malformed protein? What is the consequence of this deformity?  What are potential treatment options, and how do they address and remedy the problem?

HOW MANY DIFFERENT PROTEINS CAN BE CONSTRUCTED THAT ARE EXACTLY FOUR AMINO ACIDS LONG? (HINT: THERE ARE 20 POSSIBLE AMINO ACIDS.)