What is an advantage of using an ELISA instead of a protein microarray to study a proteome? What is a disadvantage?
Q: If you mix two unknown samples and repeat the Lowry assay, is the absorbance equivalent to the sum…
A: Lawry essay is used to determine the protein level in a solution as it shows a color change…
Q: What is ORF scanning?
A: The open reading frame(ORF) in molecular genetics is a continuous stretch of codons that begins with…
Q: What is the application of Bradford assay?
A: There are several methods to determine various biomolecules either quantitatively or qualitatively.…
Q: What is VNTR profiling, and what are the applications of thistechnique?
A: DNA profiling is a forensic technique in criminal investigations. It is used as a person's DNA to…
Q: Describe the process of doing a microarray.
A: A laboratory tool is used to measure the expression of thousands of genes at the same time and this…
Q: what is the difference between presumptive and confirmational analysis?
A: There are certain tests which requires simple techniques while other require complex experimental…
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A: In the conventional amplification technique of PCR (polymerase chain reaction), the amplified…
Q: Describe the principles behind direct and indirect fluorescent antibodytests.
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Q: At what stage of the culture should bacterial colonies be harvested for plasmid DNA extraction? How…
A: Plasmid training is a way of DNA extraction and purification for plasmid DNA. Many techniques were…
Q: Why is the DNS method used in glucose assay?
A: 3,5-dinitrosalicylic acid (DNS) measure has been broadly utilized for the estimation of diminishing…
Q: TGF-beta treatment cause cells to grow more or less in the soft-agar assay
A: TGF beta The transforming growth factor-beta is a cytokine a protein which is produced by all types…
Q: What is cell lysis? Discuss the principles behind DNA isolation (extraction and precipitation…
A: A eukaryotic cell consists of many membrane-bound organelles. The membrane-bound organelles form…
Q: Why is it essential to add sodium azide to succinate dehydrogenase assay solution? What is the…
A: In laboratories various assays are carried out for the determination of functionality of various…
Q: What do you mean by fluorescent-labeled probes?
A: Fluorescent marking is the interaction of covalently binding fluorescent colors to biomolecules,…
Q: What is the difference between in-ovo and ex-ovo types of CAM Assay?
A: The CAM test is a robust approach that can be used to analyze the involvement of new chemicals and…
Q: Explain in your own words how bacteria are grown to form the sample for the antibiotic resistance…
A: Antibiotic resistance The term antibiotic resistance is made up of two words i.e. resistance(ability…
Q: In the “sandwich” ELISA technique, what is the function of the capture antibody? To link an enzyme…
A: Immunoassay are techniques that have the capability to detect even minute quantities of antigen or…
Q: What is the principle of the gel electrophoresis separation technique?
A: Biological techniques are procedures or strategies for studying biological things. Biological…
Q: What type of probes must be used for super-resolutionmicroscopy and why?
A: The super-resolution microscopy involves a series of techniques that allow high-resolution images.…
Q: If we have different protein samples. How would we analyze them using SDS- PAGE and 2-D gel…
A: Purification of proteins is a critical step in gaining a full understanding of the protein's…
Q: The probe in the figure below can be considered as : *
A: A biomedical device is an instrument that is used in medicine for the purpose of diagnosis,…
Q: Why is the bradford assay method using BSA sensitive ? and how does it compare to the amino acid…
A: Bradford assay: It is a widely used method for measuring total protein concentration and different…
Q: How do we separate plasmid and chromosomal DNA during the alkaline plasmid screen?
A: Alkaline lysis is the most common method used for separating plasmid DNA from chromosomal DNA.
Q: Among these protein assays, which is appropriate for solutions with high protein concentration or…
A: Protein assay is a quantitative analytical technique. It is carried out to determine the amount of…
Q: What is a reason that a cut digest insert would not match up in length with a PCR insert in gel…
A: Electrophoresis describes the separation of charged particles and their migration in the influence…
Q: When developing the zymogram using a destaining solution, a researcher observed wedge shaped bands…
A: Zymography is a technique to assess the enzymatic activity of proteins either in situ or by…
Q: If a concentrated sample is out of the detection range of a chosen dye-based assay, what can be…
A: Dye based assay is a dye binding protein assay based on the binding of protein molecules to…
Q: There are two figures- one for vector control plate & another PCRinsert+vector experimental plate.…
A: We know that genetic selection of transformed or transfected cells is one of the significant steps…
Q: What is the purpose of using Triton X in hemolytic assay.
A: Hemolysis is release of hemoglobin into the blood after the red blood cells are lysed. In hemolytic…
Q: Bacterial mutants can be classified on the basis of the method, Which is those methods?
A: Microorganisms undergo mutations under environmental stress that causes sudden heritable changes in…
Q: In the plaque assay, what is the precise origin of a single plaque?
A: Answer: Introduction: Virus is an intracellular infectious parasite consist of only one type of…
Q: what are two differences between agarose gel electrophoresis and SDS PAGE?
A: Agarose gel electrophoresis and the SDS page technique are used to separate the molecules. The…
Q: In the plaque assay, exactly what makes up a single plaque?
A: Viruses are non-cellular pathogenic particles. Viruses cannot survive on their own as they lack cell…
Q: Can gel electrophoresis be a quantitative technique? How so?
A: DNA, proteins, RNA are biomolecules which are very crucial in a cell. Many different techniques are…
Q: Why is it not necessary to dilute your protein samples with buffer in a bradford protein assay…
A: This assay is used to measure the concentration of protein.
Q: State two reasons behind using the blocking buffer in ELISA experiment.
A: ELISA (Enzyme linked immunosorbent assay) is a plate based assay technique designed for detecting…
Q: What is the purpose of the Ames test? How are his− bacteria used in this test?
A: Ames test is also known as Bacterial reverse mutation test. It is a method to test a chemical…
Q: Vhat is needed from the cells for PCR?
A: The polymerase chain reaction (PCR) is a technique for replicating a piece of DNA millions of times.…
Q: How is a specific probe obtained?
A: Probe is a single-stranded sequence of DNA or RNA used to search for its complementary sequence in a…
Q: What is the difference between the total and faecal coliform
A: MPN assay is the statistical, multi-stage test for determining the presence of bacteria in water,…
Q: What is probe? Why it is used in the library screening ?
A: Biotechnology is the practice of using living organisms such as animals, plants, microbes, and…
Q: What is the basis for the technique called ELISA?
A: Engvall and Perlmann initially described the enzyme-linked immunosorbent test in 1971, and it is…
Q: What is single molecule sequencing in real time (SMRT) ?
A: DNA sequencing is a technique for determining the order of the four nucleotide bases found in DNA:…
Q: What technique is used to stain bacteria? Who developed this technique?
A: Microorganisms are such small living organisms that are less than 0.1 mm, and can be seen only under…
Q: Why do DNA polymerases fromthermophilic microbes significantly improve the PCRprocedure?
A: Step 1 :Introduction PCR has been used widely in fields such as forensics, genetic testing, and…
What is an advantage of using an ELISA instead of a protein microarray to study a proteome? What is a disadvantage?
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- In the “sandwich” ELISA technique, what is the function of the capture antibody? In the “sandwich” ELISA technique, what is the function of the capture antibody? To link an enzyme and the molecule of interest To convert a substrate from colorless to blue To block any “open spaces” on the well before adding the samples that potentially contain the molecule of interest To concentrate the molecule of interestExplain the mechanisms of the three types of ELISA we discussed in lab: 1) direct, 2) indirect, 3) sandwich. Which type of ELISA did we do in lab? What is one advantage of this kind of ELISA?What are the steps in sandwich ELISA? Explain and provide a schemiatic diagram
- When developing the zymogram using a destaining solution, a researcher observed wedge shaped bands with protein. What could be the possible reason for such an observation?Is it important to have fewer phages than bacterial host cells when doing a quantitative plaque assay? Explain.explain how the following genotypic andphenotypic methods of microbe identification is done. Use diagrams or schematic representations to explain yourwork. (1) Restriction Fragment Length Polymorphism (RFLP)(2) Pulsed-Field Gel Electrophoresis (PFGE)(3) Whole Genome Sequencing (WGS)(4) Gram Staining(5) Biochemical Reactions: Multiple Tube Fermentation Technique(6) Biochemical Reactions: IMViC Test
- Which of the following are true regarding assay attributes? List all that are true. a) Accuracy represents the closeness of the assay result to the “true value”. b) Robustness represents the ability of a method to distinguish between the analyte and similar components. c) Precision is determined by replicate analysis of a reference standard or well-characterized material. d) A robust assay would have a very narrow range of acceptable assay conditions. For those that were not true in question 1, correct the statement(s).With detail, compare and contrast the following 5 real-time assays; Taqman, SYBR Green, Molecular Beacon, FRET, and Scorpion. Please write complete sentences in paragraph form. Identify how the assays are similar but more importantly, what are the distinguishing features of each of the technologies.A high-throughput assay is being conducted in a 96 well plate to test compounds for anti-bacterial activity. Live bacterial cells are detected using “Live clear", a dye which is initially coloured blue, but turns clear in the presence of live bacterial cells. The control wells are shown below. Penicillin kills bacteria; glycerol does not affect them. Which of the control wells will appear blue at the end of the assay? "Live clear" glycerol penicillin - - + (A1 A2)(A3)A4) bacterial cells B1 (B2 (B3)B4 А4, В1, B2, ВЗ, B4 A2, A4, B2, B3, B4 А4, B2, ВЗ, В4 A1, АЗ, В1, В2, вЗ, В4 + + |