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- Which is the more reliable technique for identifying an unknown compound and why? IR spectrum Melting point Thin-layer chromostography (TLC)Describe how to prepare adulterated palm oil samples before analysing it with Raman spectrometer. Also the describe the process how the Raman spectrometer will analyze the adulterated palm oil samplesThe Beer-Lambert law you'll be using to determine the concentration of an unknown is A = ebc where A is the absorbance measured on the spectrometer, e (epsilon) is the molar absorptivity in M cm1, b is the path length of the cell (the diameter of the cuvette which is filled with solution), and c is the concentration of the solution in M. If you have a sample which has an absorbance of 1.17, a molar absorptivity of 1 Mcm1, and a cuvette that is 1.15 cm wide, what is the concentration of the sample in M? Give your answer to 3 decimal places (e.g.: 0.123)
- Here is the protocol for a UV-Vis spectrophotometer to detect water and chlorine-carbon. 1.Dissolve the water and chlorine-carbon compounds in a solvent, such as water. 2.Prepare a standard solution of known concentration that is similar to the sample being measured. 3.Calibrate the spectrophotometer using the standard solution. 4.Measure the absorbance of the sample using the spectrophotometer. 5.Calculate the concentration of the compounds in the sample using the calibration curve obtained from the standard solution. How is the spectrophotometer calibrated with standard solutions? When is the blank solution placed in the spectrophotmeter?1- While cleaning out a laboratory shelf, you have made a mistake in labeling two bottles of ethanol and acetic acid. To identify the molecular structure of the contents of the bottle, samples were submitted for analysis using infrared spectroscopy, based on the IR results, draw the molecular structure of the molecule that would correspond to the spectrum for that compound. Justify your answer 0.8 0.6- 0.4- 0.2 3000 2000 Wavenumber (cm-1) 1000 80 70 60 40 30- 20 10 2000 Wavenumbers (cm-1) II TransmitanceA student weighed out 0.150 g of protein powder and dissolved it in 100 mL of water (Solution 1). The student then diluted this solution by transferring 1 mL into a 25 mL flask and diluting with water (Solution 2). Finally, 1 mL of that solution was transferred to a test tube and combined with 4 mL Bradford reagent. The absorbance of the solution in the test tube was 0.144. Assuming that the best fit linear line of the standard curve was y=0.04144x+0.01521 (μgmL), calculate the percent protein by mass in the original protein powder.
- A student weighed out 0.150 g of protein powder and dissolved it in 100 mL of water (Solution 1). The student then diluted this solution by transferring 1 mL into a 25 mL flask and diluting with water (Solution 2). Finally, 1 mL of that solution was transferred to a test tube and combined with 4 mL Bradford reagent. The absorbance of the solution in the test tube was 0.11. Assuming that the best fit linear line of the standard curve was y = 0.04144 x + 0.01521 (μ g mL), calculate the percent protein by mass in the original protein powder.Why is it important to acquire a reference spectrum and subtract it from the sample’s spectrum? Why are quartz cuvettes better for UV-visible absorption spectroscopy than polystyrene cuvettes, especially at lower wavelengths (UV)? What would happen if you put too much of a compound in the cuvette during the spectral measurement? Why is it important to avoid any scratch on the cuvette? Could you use a compact fluorescent light (CFL) bulb to perform UV-visible absorption spectroscopy? Why/why not?The data in the table are used to create a calibration curve for the determination of RNA from its absorbance at 260 nm. Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the best straight line fit to the calibration curve. Do not subtract the blank reading when creating the calibration curve. m = b = RNA (µg) 0.00 10.67 21.34 32.01 42.68 mass= Absorbance at 260 nm 0.224 0.512 0.769 1.145 1.465 Using the slope and intercept of the calibration curve, determine the quantity of RNA in a sample that gives an absorbance of 0.636. µg µg
- Sally obtains a standard calibration curve for their assigned food dye by plotting absorbance versus concentration (in M) of known solutions and finds the following slope and intercept: y = 1.646x + 0.026 Note: Copying and/or posting this or other questions without the express written permission from Dr. Burke and the Department of Chemistry & Biochemistry at the University of Delaware is a violation of intellectual property copyright law. Sally finds that her original Kool-aid sample is too concentrated, so they dilute it by transferring 13.00 mL of the original Kool-aid sample into a new container and diluting to a total volume of 37.00 mL. If the absorbance of the dilute Kool-aid sample at the appropriate wavelength for their assigned dye is 0.950, determine the concentration (in M) of the assigned food dye in the original Kool-aid sample. Report your answer with three places after the decimal.The data in the table are used to create a calibration curve for the determination of RNA from its absorbance at 260 nm. Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the best straight line fit to the calibration curve. Do not subtract the blank reading when creating the calibration curve. m= RNA (µg) 0.00 10.29 20.58 30.87 41.16 mass= Absorbance at 260 nm 0.227 0.452 0.786 1.078 1.296 Hg-1 Using the slope and intercept of the calibration curve, determine the quantity of RNA in a sample that gives an absorbance of 0.523. µgA 0.1 g amine-containing compound is dissolved in water then diluted to 100 mL. To get the concentration of amine in this compound, you subject it to spectroscopic analysis. So, you get 1 mL of the previously diluted sample then dilute it again to 250 mL for measurement. Then, you fill 3/4 of a 1-cm cuvette with this diluted sample then you run an analysis using an AAS. The recorded absorbance is 0.545 at 410 nm. Solve for the molecular weight of the compound. The molar absorptivity is 1.23 x 104 cm-1 mol-1 L.