What can a pH-activity profile of an enzyme tell you? Select one: а. The kinetic mechanism b. How many tryptophans the enzyme has c. The entropy loss upon substrate binding O d. Residues implicated in catalysis е. The specificity of the enzyme for free hydroxide ions

Which of the followingdescribe superior properties of enzymes (biological catalysts) over traditional chemical catalysts? a. They are mostly and generally operative under mild temperature, pressure, and pH conditions b. They are regulated only by substrate concentration c. They do not effect the reaction equilibrium, but lower the reaction's activation energy d. They are recycled at the end of the reaction Choose all that apply

Describe reaction between AP and PNPP and how is used to determine the kinetic parameters of AP. i. İndicate the value for the activity of AP(which is 3.08) What does it mean? Is your enzyme active or not? What does it say about the purity of your enzyme? ii. How the purification process affects the activity?

ANSWER A AND B PLS