What are three techniques that can be used to show that the electron transport chain is found on the matrix side of the inner mitochondrial membrane. Select all that apply. O protease protection assay Ocell fractionation O immuno EM O Western blotting Olight microscopy O chemically derivatizing proteins OSDS-PAGE

6) Pore-forming toxins are a class of protein-based toxic polymers that result in the formation of large multi-protein channels that allow the free flow of substrates and liquid in and out of a cell or organelle. Research shows that these can attach to the outer mitochondrial membrane, or on the thylakoid membranes Hollyal of chloroplasts. Meman Extracelular binding space Proteiniciati Inactive soluble T obare Lipid Activation Active P Assembly Membrane tion Pelit K Partially formed pome Nunta Inson Large molecule A Protein What would the possible consequences be if this pore-forming toxin attached to the mitochondrial outer membrane or the thylakoid membrane? Explain why. ATP Nature Reviews Microbiology

Which of the following is an advantage of confocal microscopy over conventional fluorescence microscopy? The interaction of the laser beam with the cell surface allows the imaging of individual macromolecules O Only out-of-focus light enters the microscope lens, making the object appear illuminated against a dark background The use of electrons instead of light to image the specimen results in greatly increased resolving power O Optical sections in a single plane of focus can be visualized at different depths in the specimen The natural contrast of an unstained specimen is enhanced

Using Figure 2 shown below, sketch one of the patterns of fluorescence that you mightobserveifyoumeasuredtheintensityoffluorescence overthetimeittakes the labelled ATP synthase to synthesise six molecules of ATP.

label cell structures. the photo is of stained mammalian cellsthat are under under a compound light microscope at 400x total magnification. the cells were treated with cell fixing agent, methylene blue stain, and eosin stain.

sentence) 16. Use these 6 phrases and write them in the mitochondrial area that they best describe. High [H+] Higher pH Lower pH Negatively-charged Positively-charged Low [H+] Mitochondrial Matrix ANSWER ANSWER ANSWER 2 Body* #3 3 A $ 4 B % 5 Mitochondrial Intermembrane Space ANSWER ANSWER ANSWER I MacBook Pro U 6 bb & 7 8

Which of these compounds will cross the cell membrane without the aid of a membrane transport protein, and why? H3 NH NH 2. By what transport process will the above compound be transported? What are the factors which drive movement across the membrane in this process? Does this process require input of energy?

To print a medical scaffold made out of TPU to surgically implant around the pancreas for diabetic patients can you explain why Bio Printing the scaffold is better than SLA and which materials would be best to use for Bio priting out of collagen, fibrin or silk.  What are the advantages and limitations of SLA and Bioprinting.

How does uniport transport (facilitated diffusion) compare with simple diffusion? O Uniport transport is much faster and more specific than simple diffusion O Uniport transport consumes energy but simple diffusion does not O Uniport transport is slower but more specific than simple diffusion Similar to simple diffusion, uniport transport is nonspecific O None of the other are correct

Label the diagram below as either passive transport (diffusion or facilitated diffusion) or active transport. Provide examples of solutes that are transported in these various ways across the cell membrane