What are the three general procedures in subcellular fractionation?

What is Huble's Test's primary objective? How does it compare/differ from the Unsaturation Test? How are these examinations conducted? What are the affirmative outcomes of this test? What is the underlying idea of this test?

Mrs. Ho, age 50, was infected by a bacterium and was diagnosed with cystitis two months ago. Her doctor gave her some drugs but they did not work. Last week, her condition worsened, and she displayed symptoms of acute renal failure. (a)  What is cystitis?  (b)  Explain how cystitis could lead to acute renal failure in Mrs. Ho.  (c)  Suggest a possible treatment for acute renal failure. Describe how the treatment should be carried out.

Why is the risk of toxicity greater for fat-soluble vitamins than water-soluble vitamins? Question 72 options: a)  Fat-soluble vitamins are excreted in the urine along with water-soluble vitamins b)  Fat-soluble vitamins are stored in the liver while water-soluble vitamins are not c)  Unlike water-soluble vitamins, fat-soluble vitamins are not readily excreted and can accumulate in tissue d)  Fat-soluble vitamins, in supplement form, are more bioavailable, compared to water-soluble vitamins