What are the main differences between bottom up/shotgun and top down proteomics strategies?

Q: How does metaproteomics differ from proteomics?

A: Proteins are known as building blocks of the body. They are made up of long chain of amino acids…

Q: What is the functional difference between regular PCR and qPCCR?

A: PCR stands for a polymerase chain reaction. As the name suggests that polymerase chain reaction…

Q: what is the whole-genome shotgun sequencing? Also briefly explain its strategy to assemble the…

A: Whole - genome shotgun sequencing is a single step sequencing method, in which the genome is broken…

Q: How do the individual protein techniques combine to study proteomics?

A: Proteins are organic macromolecules that make up around 50% of cellular dry weight. Proteins are…

Q: How does an RT-PCR work?

A: RT- PCR is a technique used in genetic studies used for the detection and quantification of mRNA.…

Q: What are the emerging techniques and challenges in utilizing multi-omics data for comprehensive…

A: The rapid advancements in sequencing technologies have allowed us to generate a wealth of data on…

Q: How do shotgun and directed sequencing differ? Why is coverage an important issue?

A: Shotgun sequencing and directed sequencing are two different approaches in the field of DNA…

Q: What are some of the technical reasons why gene therapy is difficult to carry out effectively?

A: Gene therapy is a technique or procedure through which a faulty gene or non functional gene can be…

Q: There are four possible observations that suggest an error occurred in a PCR reaction: a) Sequence…

A: PCR reaction is a method used to amplify a small sample into millions of small ones which help the…

Q: At the present level of advancement of biotechnology, what are the main techniques of genetic…

A: Introduction In today's world, the most common genetic engineering techniques are: recombinant DNA…

Q: What is the difference between PCR and real-time PCR, and how do you explain it?

A: The Polymerase chain reaction (PCR) is a technique that allows researchers to rapidly make millions…

Q: What are the possible bioethical issues that gene editing tools may encounter?

A: Gene editing tools are used to edit an organisms genetic code , i.e.. with these tools ,the genetic…

Q: How does high throughput screening work?

A: High-throughput screening (HTS) is a automated process to test large numbers compounds (chemical or…

Q: What is vector? Discuss 2 vectors that are commonly used in DNA recombinant technology.

A: Answer-  Vector- vector is a DNA molecule that is used to carry a foreign DNA into the host cell. It…

Q: What is the importance of nanomedicine in biotechnology?

A: Nanomedicine means applying the nanotechnology in medical field. It can be used as a knowledge or…

Q: What is the purpose of shotgun sequencing?

A: Deoxyribonucleic acid (DNA) sequencing is the process of determining the nucleic acid sequence based…

Q: Explain how DNA microarrays are used in the study ofgenomics.

A: DNA (deoxyribonucleic acid) is double helix, coil structure that is responsible for transfer of…

Q: How can site-directed mutagenesis be useful to enzymologists?

A: Mutagenesis is the biological process of changing genetic information or DNA of an organism and that…

Q: what are similarities and differences between chain-termination and reversible terminator…

A: Gene sequencing  aids in the development of gene therapy, a form of treatment that replaces faulty…

Q: What is the function of the CRISPR/Cas system? What are the ethical impacts of a technology such as…

A: Genetic and molecular biology is the study of genetic information such as the genetic material,…

Q: What are the potential advantages of promoting artificialphotosynthesis research?

A: Artificial photosynthesis is a process of an artificial method of copying the natural process of…

Q: Although a number of different animals have been successfully cloned, the process of creating cloned…

A: A living organism, which is genetically identical to that of the other organism is known as a Clone.…

Q: Who developed the sequencing-bysynthesis (SBS) approach? & When it was firstly used ?

A: Sequencing by synthesis (SBS), an Illumina sequencing method, is a commonly used next-generation…

Q: What are the different types of PCR and their product concepts? Site and explain the comparison…

A: Introduction :- Polymerase Chain Reaction (PCR) is a molecular biology technique for making multiple…

Q: What are the reasons why there needs to be more than 10 cycles in the PCR process? Name 2 reasons.

A: PCR (Polymerase Chain Reaction) is a technique used to amplify DNA sequences in vitro. The process…

Q: Give the different uses of proteomics in the field of genetics.

A: The nucleus of the cell contains DNA on which coding sequences known as genes are located. The genes…

Q: Genetic engineering and gene therapy are two issues in genomics that are quite similar. What…

A: Studying an organism's full genetic complement is known as genomics (the genome). It uses…

Q: How does the shotgun approach differ from the whole genome sequencing?

A: Biotechnology is concerned with exploitation of biological agents or their components for generating…