Enzyme kinetics
In biochemistry, enzymes are proteins that act as biological catalysts. Catalysis is the addition of a catalyst to a chemical reaction to speed up the pace of the reaction. Catalysis can be categorized as either homogeneous or heterogeneous, depending on whether the catalysts are distributed in the same phase as that of the reactants. Enzymes are an essential part of the cell because, without them, many organic processes would slow down and thus will affect the processes that are important for cell survival and sustenance.
Regulation of Enzymes
A substance that acts as a catalyst to regulate the reaction rate in the living organism's metabolic pathways without itself getting altered is an enzyme. Most of the biological reactions and metabolic pathways in the living systems are carried out by enzymes. They are specific for their works and work in particular conditions. It maintains the best possible rate of reaction in the most stable state. The enzymes have distinct properties as they can proceed with the reaction in any direction, their particular binding sites, pH specificity, temperature specificity required in very few amounts.
What are the extinction coefficient of Myoglobin and BSA at 595nm respectively?
Hb is present in erythrocytes, while Mb is found in the muscle tissues. Both the proteins are composed of protein part (globin) and non-protein part (heme). Heme consists of an iron molecule, which is attached to a porphyrin compound. It is an oxygen-binding molecule that ensures the transportation of oxygen in the bloodstream.
Hb and Mb are significant oxygen-transporters molecules. Oxygen binds reversibly to iron atoms present in these proteins. The bond between myoglobin and oxygen is stronger than the bond between Hb and oxygen. The “oxygen-loaded” forms of Hb and Mb are called oxyhemoglobin and oxymyoglobin, respectively.
Step by step
Solved in 2 steps
