Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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Question

What are the extinction coefficient of Myoglobin and BSA at 595nm respectively?

Expert Solution
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Hb is present in erythrocytes, while Mb is found in the muscle tissues. Both the proteins are composed of protein part (globin) and non-protein part (heme). Heme consists of an iron molecule, which is attached to a porphyrin compound. It is an oxygen-binding molecule that ensures the transportation of oxygen in the bloodstream. 

 

Hb and Mb are significant oxygen-transporters molecules. Oxygen binds reversibly to iron atoms present in these proteins. The bond between myoglobin and oxygen is stronger than the bond between Hb and oxygen. The “oxygen-loaded” forms of Hb and Mb are called oxyhemoglobin and oxymyoglobin, respectively. 

 

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