What are the differences between preparing an RNA sample for sequencing and a genomic DNA sample?

Q: What is one major way in which next generation sequencing differs from dideoxy sequencing (Sanger)?

A: The technique of sequencing DNA involves identifying the nucleic acid sequence, or the arrangement…

Q: Why is genome database and annotation relevant to Genome sequencing?   Are there any examples?

A: Genome: the set of genes. Genome database: it is the collection of all information on the human…

Q: Describe the shotgun method for sequencing an entire genome.

A: Genome or genetic material of a cell is the DNA content present in the nucleus. DNA or…

Q: What is the importance of Recombinant DNA Technology in the Molecular Laboratory

A: Recombinant DNA technology- Recombinant DNA technology is the joining of two different DNA molecules…

Q: What is exome sequencing used for?

A: DNA sequencing:   It is a method to find a sequence of nucleotides by using the DNA sequencing…

Q: How do shotgun and directed sequencing differ? Why is coverage an important issue?

A: Shotgun sequencing and directed sequencing are two different approaches in the field of DNA…

Q: Outline the method of RNA sequencing (RNA-Seq).

A: Ribonucleic acid is a type of nucleic acid majorly involved in the gene expression, gene regulation…

Q: Describe the technology behind identifying, synthesizing,sequencing, and amplifying DNA.

A: Deoxy ribonucleic acid (DNA) is the genetic material of most organisms that carry coded genetic…

Q: What are the events in the following phases of Transcription: Initiation, Elongation and…

A: Transcription is the process of generating RNA from DNA. Gene expression is another name for it.…

Q: Describe the composition of a DNA microarray, and explain how it is used.

A: A DNA microarray is a laboratory tool which is used to detect the expression of thousands of genes…

Q: How accurate is whole genome sequencing?

A: The genome of an organism is defined as the whole heredity information encoded in the genetic…

Q: Discuss the significance of protein sequencing.

A: Protein sequencing is a process of determining the sequence of amino acids of a protein.

Q: What is hierarchical sequencing?

A: Basically there are two approaches for the sequencing of large complex genome. They are hierarchical…

Q: What are the advantages of using a recombinant DNA product from a human source instead of a product…

A: GENETIC ENGINEERING:- It is a modification in genes that is done using biotechnology ( Recombinant…

Q: What are techniques available for Analyzing DNA ?

A: Techniques which are availaible for Analyzing DNA are :   By the use of Methylation and Sensitive…

Q: Explain the methods of DNA and RNA analysis ?

A: Introduction  Genome consists of DNA/RNA which consists of nucleotides either deoxyribose…

Q: What are gene microarrays? How are microarrays used?

A: The process of forming a single double-stranded nucleic acid molecule by combining two…

Q: What provides a convenient bridge between the low resolution of a karyotype and the ultra-high…

A: Introduction  Cytology refers to the study of cell such as cell morphology, physiology etc. As we…

Q: Discuss the concept of the use of DNA in genome identification.

A: DNA (Deoxyribo nucleic acid) is a double stranded genetic element in all prokaryotic and eukaryotic…

Q: How to create DNA-sequence alignment ?

A: DNA-Sequence alignment can be defined as the way in which sequences of DNA samples are arranged for…

Q: How is recombinant DNA inserted into a genome using CRISPRediting?

A: The modern technology of genetics can be utilized to increase the genomes of various living…

Q: What is the purpose of shotgun sequencing?

A: Deoxyribonucleic acid (DNA) sequencing is the process of determining the nucleic acid sequence based…

Q: What is oligonucleotide microarray?

A: An array is an orderly arrangement of samples where matching of known and unknown DNA samples is…

Q: Explain how DNA microarrays are used in the study ofgenomics.

A: DNA (deoxyribonucleic acid) is double helix, coil structure that is responsible for transfer of…

Q: What are possible mistakes that can cause the DNA to be contaminated during isolation, purification…

A: Various techniques are used to extract different types of DNA. Cells are broken open using a…

Q: How to use a microarray for transcriptome analysis ?

A: Transcriptome is the complete set of the mRNA molecules expressed in an individual . It is termed as…

Q: what are similarities and differences between chain-termination and reversible terminator…

A: Gene sequencing  aids in the development of gene therapy, a form of treatment that replaces faulty…

Q: What is the function of the CRISPR/Cas system? What are the ethical impacts of a technology such as…

A: Genetic and molecular biology is the study of genetic information such as the genetic material,…

Q: Explain the sequencing-by synthesis (SBS) approach ?

A: One of the well-known states that are known as fundamental topics in Arithmetic is sequence and…

Q: Who developed the sequencing-bysynthesis (SBS) approach? & When it was firstly used ?

A: Sequencing by synthesis (SBS), an Illumina sequencing method, is a commonly used next-generation…

Q: analyzing sequencing data, what parameters should be considered?

A: Genomic research has been transformed by improvements in DNA sequencing technology, which have made…

Q: What are some possible research questions and practical applications that could be addressed by…

A: The engineering and manipulation of an organism’s genetic material on the scale of entire genome…

Q: Why does one need a vector to clone a DNA

A: Cloning is the method of generating the number of copies of desired DNA I  the host.

Q: Outline different strategies for identifying gene sequences.

A: Genome is the study of all genes present in the organism. Gene is a unit of heredity that is…