What are the differences between preparing an RNA sample for sequencing and a genomic DNA sample?

Q: Describe several applications of DNA fi ngerprinting and microarray analysis.

A: DNA fingerprinting: - It is a method which provide genetic information of any living thing. It…

Q: what is the whole-genome shotgun sequencing? Also briefly explain its strategy to assemble the…

A: Whole - genome shotgun sequencing is a single step sequencing method, in which the genome is broken…

Q: What is one major way in which next generation sequencing differs from dideoxy sequencing (Sanger)?

A: The technique of sequencing DNA involves identifying the nucleic acid sequence, or the arrangement…

Q: Describe the shotgun method for sequencing an entire genome.

A: Genome or genetic material of a cell is the DNA content present in the nucleus. DNA or…

Q: How do shotgun and directed sequencing differ? Why is coverage an important issue?

A: Shotgun sequencing and directed sequencing are two different approaches in the field of DNA…

Q: Outline the method of RNA sequencing (RNA-Seq).

A: Ribonucleic acid is a type of nucleic acid majorly involved in the gene expression, gene regulation…

Q: What benefits are there when using a DNA microarray over a genetic marker such as a STR?

A: The term genetic refers to the DNA molecule present in the nucleus of cells. DNA or deoxyribonucleic…

Q: Describe the automated dideoxy chain termination method of DNA sequencing.

A: The chain termination DNA sequencing method is the Sangers sequencing in which the DNA fragment is…

Q: Why are entire genomes not used for DNA profiling?

A: DNA profiling is the process of characterizing a sequence of DNA obtained from a person. It is also…

Q: Describe the composition of a DNA microarray, and explain how it is used.

A: A DNA microarray is a laboratory tool which is used to detect the expression of thousands of genes…

Q: How is DNA used to make a profile and how is the profile used?

A: The abbreviation DNA stands for deoxyribonucleic acid. DNA is found to be the hereditary material in…

Q: Explain the methods of DNA and RNA analysis ?

A: Introduction  Genome consists of DNA/RNA which consists of nucleotides either deoxyribose…

Q: What is a repetitive element in genomics?  What are the types of repetitive elements?  What is their…

A: The amount  of DNA present in a cell of a person is called as genome. DNA is made up of nucleotides…

Q: What provides a convenient bridge between the low resolution of a karyotype and the ultra-high…

A: Introduction  Cytology refers to the study of cell such as cell morphology, physiology etc. As we…

Q: How to create DNA-sequence alignment ?

A: DNA-Sequence alignment can be defined as the way in which sequences of DNA samples are arranged for…

Q: Explain the difference between sequencing by chain termination and sequencing by synthesis. Which…

A: Chain termination process employs dideoxynucleoside triphosphates (ddNTPs) lacking OH (hydroxyl)…

Q: What is the purpose of shotgun sequencing?

A: Deoxyribonucleic acid (DNA) sequencing is the process of determining the nucleic acid sequence based…

Q: What is oligonucleotide microarray?

A: An array is an orderly arrangement of samples where matching of known and unknown DNA samples is…

Q: What are possible mistakes that can cause the DNA to be contaminated during isolation, purification…

A: Various techniques are used to extract different types of DNA. Cells are broken open using a…

Q: Is it necessary to cut the isolated genomic DNA of an organism with restriction enzymes for…

A: Restriction enzymes are also known as DNA-cutting enzymes which recognizes one or a few target…

Q: How to use a microarray for transcriptome analysis ?

A: Transcriptome is the complete set of the mRNA molecules expressed in an individual . It is termed as…

Q: Define the process of Interpreting DNA Profiles ?

A: DNA profiling is also called DNA fingerprinting or DNA typing. It is a technique used to distinguish…

Q: Explain the sequencing-by synthesis (SBS) approach ?

A: One of the well-known states that are known as fundamental topics in Arithmetic is sequence and…

Q: analyzing sequencing data, what parameters should be considered?

A: Genomic research has been transformed by improvements in DNA sequencing technology, which have made…

Q: Why does one need a vector to clone a DNA

A: Cloning is the method of generating the number of copies of desired DNA I  the host.

Q: Outline different strategies for identifying gene sequences.

A: Genome is the study of all genes present in the organism. Gene is a unit of heredity that is…

Q: Explain the use of a probe in screening a DNA library

A: Introduction: Probes are the small DNA or RNA strands that are used to label DNA. The labeling of…

Q: What are the major differences between dna probe and Primer

A: DNA as a genetic material in all the living organisms.

Q: What are some difficulties with low copy number DNA analysis? What kinds of checks and balances can…

A: INTRODUCTION DNA analysis DNA analysis is a method of analyzing the characteristics of DNA. It is…

Q: How can specific genes be retrieved from a DNA library?

A: Deoxyribonucleic acid or DNA is a molecule that contains the genetic code of the organisms. DNA…

Q: What is DNA fingerprinting? Define and differentiate VNTR and Probe and what is the applications of…

A: Step 1 DNA or deoxyribonucleic acid occurs in living systems and can function as genetic materials.…

Q: How does the shotgun approach differ from the whole genome sequencing?

A: Biotechnology is concerned with exploitation of biological agents or their components for generating…

Q: Outline the steps in automated DNA sequencing via the dideoxy method.

A: DNA sequencing:   It is a method to find a sequence of nucleotides by using the DNA sequencing…

Q: What is the major difference between the strategies of map based sequencing and shotgun sequencing?

A: DNA sequencing is a process in which sequence(order) of nucleotides in DNA is determined. It…