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1. What are restriction enzymes.
2. Give 5 restriction enzymes and its source.
3. What is a restriction map.
4. Explain each step used in restriction mapping.
STEP 1: Preparation of DNA for restriction analysis.
STEP 2: Restriction digestion of DNA.
STEP 3: Separation of restricted DNA.
STEP 4: Collecting data.
STEP 5: Construction of restriction map
Step by step
Solved in 2 steps
- 1. What is the purpose of restriction enzyme? 2. What is the purpose of the probe in DNA finger printing. 3. What is/are the sample that can be used for DNA finger printing? 4. Who is the culprit base on our interactive laboratory experiment?Part A. If student counts 63 colonies on their 10^-6 dilution LB plate. What was the original concentration of their cells if they plated 100ul? Part B.If we used pGFPuv as the template for PCR positive control. This is because: a. it contains the GFP gene so it should show a product. b. It contains DNA fragments that were added to the ligation reaction. c. It is the desired plasmid we wanted to make. d. So we have a band to compare our unknown plasid to allowing us to check if the unknown is the right size.Which statement is NOT the function of restriction enzyme? Group of answer choices It cuts the vector’s plasmid and the desired gene from the original DNA. It ligates the desired to gene to vector’s plasmid. It acts as scissors of DNA that cut along a specific sequence. It serves as degrading proteins that cut plasmid on specific target site.
- The other options are: a. RNA cannot be digested by restriction enzymes b. RNA is small enough to be resolved on an agarose gel without the need for restriction digestion. c. RNA is single stranded and DNA is double strandedThe segment of DNA shown in the figure has restriction sites I and II, which create DNA restriction fragments A, B, and C. Which of the gels produced by electrophoresis best represents the separation and identity of these fragments? Reminder: a positive electrode is located at one end of the gel and the negative electrode at the other end. II O a. From the positive to the negative end, the order of the fragments will be B, A, C O b. From the positive to the negative end, the order of the fragments will be A, B, C O c. From the negative to the positive end, the order of the fragments will be B, A, C O d. From the positive to the negative end, the order of the fragments will be C, A, B O e. From the negative to the positive end, the order of the fragments will be A, B, CRestriction maps illustrate the lengths of DNA fragments between restriction sites. Which of the following information can be gathered from the analysis of restriction maps? Select all that apply. a gene sequence b presence of mutations c nucleotide sequence d disease identification
- All of the following are performed during restriction fragment length polymorphism analysis. 1. splitting of double-stranded into single-stranded DNA 2. gel electrophoresis 3. autoradiography 4. immersion in radioactive probes 5. digestion of DNA with restriction endonucleases 6. use of a positive charge to transfer single-stranded DNA from a gel to a membrane. The correct sequence of these operations is whatThe main enzymes commonly used in genetic engineering are: restriction endonuclease and ligase ligase and polymerase restriction endonuclease and polymerases O endonuclease and ligaseOn the gel shown below are four DNA samples. Samples A to C are taken from tissues of landslide victims that are being identified, while sample D came from a hair sample brought by a mother looking for the remains of her son. (see img) i. If similar band patterns in a gel are created using the same restriction enzyme, what does that tell you about the DNA sequence of the samples? ii. In sample C, only two fragments were created. How many restriction sites (regions where enzymes cut) are present in sample C?
- See the restriction enzyme map below. The total DNA length is 1800 base pairs. If this DNA is cut using three restriction enzymes, namely Kpnl, Sall and EcoRI, it yields four fragments with sizes of 390 bR. 810 bp, 270 bp www and 330 bp. Kpnl Sall EcoRI 390 810 270 330 1800 bp 1. If you were to subject this digested DNA to agarose gel electrophoresis, what would your gel look like? Draw a detailed picture of your gel. Remember to indicate the direction in which your DNA is moving and also show any reference samples. Also remember to show all components of your gel. 2. You are provided with coiled DNA and plasmid DNA that you subject to gel electrophoresis. Draw this gel. Remember to indicate the direction in which your DNA is moving and also show any reference samples. Also remember to show all components of your gel. Exac fragment sizes are not important.CHOICES: a. Plasmid b. Sticky end c. DNA ligase d. Transformation e. Restriction enzyme f. Genetic marker g. Transduction QUESTIONS: Cuts the DNA into fragments Circular DNA molecule of bacteria Used to insert DNA of interest to vector Area of DNA where bases are ready to be paired Recombinant DNA technology with the help of a vector geneHow many statements are right? 1. operator is a protein (transcription factor) that interacts with a DNA sequence immediately downstream the promoter region 2. Type I restriction endonucleases cleave DNA at specific sites, close to recognition sequence 3. Type II restriction endonucleases cleave DNA within or at short specific distances from the recognition site 4. Type IIl restriction endonucleases Cleave DNA at random sites, cleave DNA near the recognition sequence 5. Type IV restriction endonucleases: Target only methylated DNA 3. 1 2. 4. 5.