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What are density-gradient centrifugation
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- What is density-gradient centrifugation? What are its advantages and disadvantages in subcellular fractionation?Describe in full the technique of density gradient centrifugation?Differential centrifugation can be improved through the use of a density gradient. What is the purpose of this, and how does it work?
- What is the similarities of differential centrifugation and gradient centrifugation?Use the following passage to answer 2 consecutive questions. A urine specimen was collected in a container and placed on the central desk for delivery at 10:00 am. The specimen is not sent to the lab until 5 hours later (3:00 pm). The sample time was marked by the central clerk as collected at 4:00 pm. The bacterial species has a generation time of 30 minutes at room temperature. There are 100 bacterial cells/ml at the time of collection (10:00 am). The laboratory will cite a bacterial infection at more than 1000 bacterial cells/ml.Which of the following is the best definition of generationtime?(a) The length of time it takes for lag phase to occur(b) The length of time it takes a population of cells to double(c) The minimum length of time it takes a cell to divide(d) The length of time a culture stays in stationary phase(e) The length of time it takes log phase to occur
- What is a Conditioned cell-free medium(CCFM)?How does subcellular centrifugation exactly work? At slower speeds, do the smaller/less dense objects form a pellet first? How do differing speeds lead to different pellet formations?Suppose that you were tasked with 4 cultures of a specific Bacteria Species in Luria Broth medium: (1) culture A – cells are in lag phase; (2) culture B – cell in log phase; (3) culture C – cells in stationary phase; and culture D – cells in decline phase. Imagine that you observe the growth rates of each culture in a fresh sterilized LB medium. Now, plot the outcome growth curves of cultures A, B, C and D in a single graph.
- In the experiment performed by Messelson and Stahl, bacterial cells grown in 15N-containing growth medium for many generations were then allowed to grow in 14N-containing medium. Suppose they would have conducted the experiment the other way around: that is, to first grow bacterial cells in 14N-containing medium for many generations and then switching them to a 15N-containing growth medium. After two generations of growth in 15N media the results of a CsCl density gradient centrifugation of the DNA would be expected to show O one band of intermediate density and one band of high-density (i.e. heavy band) one band on intermediate density O one band of intermediate-density and one band of low-density (i.e. light band) one band of low density (L.e. light band) and one band of high density (1.e. heavy band). one band of low density (I.e. light band), one band of intermediate density, and one band of high density (i.e. heavy band).What are the advantages and disadvantages of differential centrifugation?In the experiment performed by Messelson and Stahl, bacterial cells grown in 15N-containing growth medium for many generations were then allowed to grow in 14N-containing medium. Suppose they would have conducted the experiment the other way around: that is, to first grow bacterial cells in 14N-containing medium for many generations and then switching them to a 15N-containing growth medium. After two generations of growth in 15N media the results of a CsCl density gradient centrifugation of the DNA would be expected to show one band of intermediate-density and one band of low-density (i.e. light band) one band of low density (i.e. light band), one band of intermediate density, and one band of high density (i.e. heavy band). one band of intermediate density and one band of high-density (i.e. heavy band) one band on intermediate density one band of low density (i.e. light band) and one band of high density (i.e. heavy band). O O