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Introduction:-
- The lac operon, short for lactose operon is a series of three genes in bacteria that produce the necessary enzymes to obtain energy from lactose.
- Typically, glucose is the sugar of choice for bacterial organisms,but when this supply runs low, the lac operon allows the organisms to survive on lactose.
Step by step
Solved in 2 steps
- High levels of glucose in the environment result in low levels of transcription of the lac operon. The reason for this is glucose raises the levels of CAMP, which binds to the CAP protein to cause transcription repression glucose prevents lactose from binding to the Lac Repressor glucose lowers the levels of CAMP, which is necessary to activate the CAP protein glucose binds to the Lac Repressor to cause it to bind to the operator site (lac O) glucose lowers the levels of the CAP protein, which is necessary to stimulate transcription of the lac operonMatch the nutrient environment with the status of the transcription factors that regulate the lac operon in E. coli. Lacl bound to lactose and CAP is bound V[Choose ] Low lactose, low glucose High lactose, high glucose High lactose, low glucose Low lactose, high glucose CAMP and the CAP-O Lacl bound to the LacO and CAP bound CAMP and the CAP-O Lacl bound to lactose and CAP unbound [ Choose ] by CAMP, not on the CAP-O Lacl bound to the LacO and CAP unbound [ Choose ] by CAMP, not on the CAP-OThe molecules indicated below can be used experimentally due to their roles in relation to the expression of the lac operon. Drag the listed molecules to their corresponding bin. Reset Help ONPG IPTG X-Gal allolactose Dragable beta-galactosidase cleaves this molecule to form a visible yellow product. beta-galactosidase cleaves this molecule which then dimerizes to form a visible blue product. This molecule induces the transcription of the lac operon by inactivating the lac repressor.
- For the lac operon, when glucose is absent and lactose is present ___________ None of the options cAMP-CAP is bound to the operator and the repressor is not bound to the promoter cAMP-CAP is not bound to the promoter and the repressor is bound to the operator cAMP-CAP is bound to the promoter and the repressor is not bound to the operatorIn the presence of lactose and the presence of glucose the lac operon is V [ Select ] Lacl is [ Select ] t. expressed at basal levels strongly expressed to the CAP binding site. not expressedWhich of the following conditions is most likely to result in an increase rate of transcription of the lac operon? when lactose is present AND the repressor is permanently attached to the operator when lactose is present and there is high levels of glucose when lactose is absent and there are low levels of glucose when lactose is present and there are low levels of glucose
- In the absence of lactose and the presence of glucose the lac operon is strongly expressed Lacl is not bound bound to the CAP binding site. to the operator and CAP isGiven the Table below concerning the lac operon, match the letter with what would be occurring for each scenario. Repressor is bound to the operator, cAMP complex is bound and transcription is prevented. Allolactose is bound to the repressor, cAMP complex is bound, and transcription is prevalent. Allolactose is bound to the repressor, cAMP complex is not assembled, and transcription is slow Repressor is bound to the operator, cAMP complex is not assembled, and transcription is prevented.Match each description with the appropriate label (allo)Lactose binds to the lac repressor, causing it to release from the promoter CAP binds CAMP to stimulate transcripti of the lac operon ✓ [Choose ] positive control of an inducible gene negative control of an inducible gene [Choose]
- Mutations in the genes of the lac operon might affect the regulation of β-galactosidase synthesis. For each mutation listed below, indicating whether β-galactosidase would be regulated normally, always ON or always OFF. Mutation in operator site prevents repressor binding Mutation in lacIgene prevents repressor from binding operator Mutation in lacIgene prevents repressor from binding allolactose Nonsense mutation in lacZgenereWhich of the below conditions would result in the lowest level of expression from the lac operon? High lactose, low glucose, mutation in lac repressor so that it can bind the operator and lactose at the same time High lactose, high glucose, mutation in sigma 70 protein that increases its affinity for binding the promoter No lactose, deletion of the operator sequence, low glucose High lactose, high glucose, changing the -35 and -10 region of the promoter to the consensus sequence for sigma 70 binding High lactose, high CAMPThe lac operon contains three genes: lacZ, lacY, and lacA for ß-galactosidase, galactoside permease, and thiogalactoside transacetylase, respectively. The operon also contains a promoter site and an operator site. The regulatory gene includes the I gene for the Lac repressor protein and a promoter for that gene. regulatory gene DNA P₁ lacl gene The lac operon undergoes negative regulation. In the normal condition, the Lac repressor protein is active. Allolactose, an isomer of lactose, is the signal molecule that binds to the Lac repressor. P O Increase in gene products The I gene is disabled. Lactose is present in the cell. The Lac repressor dissociates from DNA. lac operon lacZ gene Determine which events will increase the concentration of lac gene products and which will decrease the concentration of lac gene products. Allolactose is not present in the cell. lacy gene lacA gene Decrease in gene products Allolactose binds to the Lac repressor protein. The Lac repressor remains bound…