Upon mixing CFP-a-p34 with GFP-a-p34 and 3-p34, the fluorescence of CFP drops from 100,000 to 96,000. When CFP-a-p34 is mixed with GFP-B-p34 in the presence of 0.5M GdnHCl the fluorescence of CFP drops from 100,000 to 63,900. If the Förster radius for the donor/acceptor pair is 50Å, how far apart are the donor and acceptor in each case?
Upon mixing CFP-a-p34 with GFP-a-p34 and 3-p34, the fluorescence of CFP drops from 100,000 to 96,000. When CFP-a-p34 is mixed with GFP-B-p34 in the presence of 0.5M GdnHCl the fluorescence of CFP drops from 100,000 to 63,900. If the Förster radius for the donor/acceptor pair is 50Å, how far apart are the donor and acceptor in each case?
Principles of Modern Chemistry
8th Edition
ISBN:9781305079113
Author:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler
Publisher:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler
Chapter20: Molecular Spectroscopy And Photochemistry
Section: Chapter Questions
Problem 6P
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