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- 1. Use the following Absorbance Data to generate a Bradford Assay Standard Curve. Protein Concentration Absorbance at 595nm (mg/mL) 0.05 Chart Title 0.0092 0.1 0.02 0.2 0.04 0.3 0.112 0.4 0.167 0.5 0.1980 0.6 0.2436 Absorbance at 595nm 0.3 4y = 0.4499x - 0.0254 R? = 0.9824 0.25 0.2 0.15 0.1 0.05 0.1 0.2 0,3 0.4 0,5 0.6 0.7PBL 1: An enzyme with a Km = 1 X 102 M was assayed to convert the substrate with initial co ncentration of 3 X 10$M. Find the time required to convert 70% of substrate when Vmax = 2.167 M/min. Km In So 1 - (So – S) t = Vmax S' Vmax S = So - xS,(Please answer all questions) 5-33. What are some advantages of using column chromatography to purify reaction products in the microscalelaboratory? 5-34. Discuss the similarities and dissimilarities of TLC, paper, and column chromatography. 5-35. Discuss the similarities and dissimilarities of HPLC and gas chromatography. 5-36. (a) What are the main advantages of using flash chromatography?(b) How can TLC be used in connection with flash chromatography? 5-37. Using the information presented on the right, please identify and explainwhich spot has an Rf value of 0.5.
- The concentration of purified OXA-M290 is tested with a BCA assay. Serial dilutions of a bovine serum albumin (BSA) stock solution are prepared, then pipetted into a 96-well plate; each dilution of the BSA standard is tested in triplicate. Then, bicinchoninic acid and Cu2+ ions are added to all of the wells of the plate. After incubating the plate for 1 hour, a microplate reader is used to measure the absorbance of all of the wells in the plate at 560 nm. This generates the following data: BSA conc. (μg/mL), Replicate 1 Absorbance, Replicate 2 Absorbance, Replicate 3 Absorbance 40, 1.360, 1.403, 1.481 20, 0.750, 0.745, 0.810 10, 0.380, 0.344, 0.398 5, 0.198, 0.160, 0.183 2.5, 0.090, 0.100, 0.085 1.25, 0.038, 0.043, 0.051 0.625, 0.024, 0.028, 0.019 Prepare a calibration curve using these data. You can use Excel, R, SPSS or an equivalent graphing software. In this graph, plot absorbance (y-axis) against the concentration of the protein standard (x-axis). Calculate and plot…Give the advantages and disadvantages of sequential injection analyzers compared to traditional flow Injection analyzers.percentage purity of an ibuprofen sample was determined by a reversed phase HPLC method as follows: Y Der ● A calibration curve of peak area versus concentration (ug/mL) of pure ibuprofen was constructed The ibuprofen sample for analysis was prepared by taking 10.0204 g of powder and dissolving it in/1Lbf distilled water (solution A). 1 ml of the latter solution was further diluted to IL with distilled water (solution B). Solution B was analysed by HPLC analysis Results The equation for the best-fit line of the calibration curve is: y=26820x-1323.8. ● The average peak area for the ibuprofen sample (solution B) was 266796.2 Calculate the percentage purity of the ibuprofen powder.
- You have a treatment process in which a microbial population consumes a substrate (chemical A) according to Monod kinetics, with Hmax=2 d', Ks = 350 mg/L, and Y 0.15 g cells/g A. What biomass concentration (in mg/L) must be maintained to result in a substrate utilization rate of -1000 mg/L- hr for a concentration of A equal to 5000 mg/L? %3D %3!In quality management, it is very important that you have a good background knowledge in both descriptive and inferential statistics. Since you already took up Biostatistics and Epidemiology during your Second Year, answer BRIEFLY the following questions on basic statistics: 1. Compare and contrast the three most common measures of central tendency mean, median and mode. 2. Differentiate standard deviation from coefficient of variation. 3. What is T-test?2 (1) When performing chemical measurements, replicate experiments are often needed. This is to mitigate (Systematic or Random) error. (2) A CHEM 215 student performed 5 measurements of the wt% of SO4²- of a solid power sample. The results are 8.75%, 8.85%, 8.89%, 8.71%, 8.64%. The standard deviation is 0.10%. What is the confidence interval for this student's experiment at 95% confidence level. The t values for 95% confidence for degree of freedom from 1 to 5 are 12.71, 4.303, 3.182, 2.776, 2.571, respectively. Show your work!
- h) i) j) Ph + НО OSI(CH3)3 Br PPH3 1) "BuLi /THF/rt / 1.5 h H CH3 homo /rt / 1 h 2) -Si(CH3)3 OCH3 TiCl4 CH₂Cl₂/-78°C / 15 min PPh3/1₂ (1 eq) imidazole / THE 0°C-itBr &. ONa DMF aprotic 2° 4 Swk or Sw2 ?I had used the E= Ecell - (RT/ nF ) InQ and got that n=2 I'm not sure what I'm doing wrong. Thanks!