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two chromatograms after LC separation. Which chromatogram is better to proceed for MS analysis? What mobile phase condition/composition (LC separation) was used?

two chromatograms after LC separation. Which chromatogram is better to proceed for MS analysis? What mobile phase condition/composition (LC separation) was used?

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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two chromatograms after LC separation. Which chromatogram is better to proceed for MS analysis? What mobile phase condition/composition (LC separation) was used? 

HPLC: 15% H₂O, 85% CH3CN, C18 Reverse Phase, 100 mm long, 5 um particles 2006 (1) 1500 Area (1)= 14750* Area (2)=53538* 1000 500 6.5 7 7.5 8 8.5 9 9.5 10 0 0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5 6 Retention Time (min) HPLC: 55% H₂O, 45% CH,CN, C18 Reverse Phase, 100 mm long, 5 µm particles 250 (2) 200 Area (1) -2256 Area (2)=9536 Area (3)=25638 150 N 100 50 ^ 0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5 6 6.5 7 7.5 8 8.5 9 9.5 10 Retention Time (min) Signal (arb, units) Signal (arb. units) 0
Transcribed Image Text:HPLC: 15% H₂O, 85% CH3CN, C18 Reverse Phase, 100 mm long, 5 um particles 2006 (1) 1500 Area (1)= 14750* Area (2)=53538* 1000 500 6.5 7 7.5 8 8.5 9 9.5 10 0 0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5 6 Retention Time (min) HPLC: 55% H₂O, 45% CH,CN, C18 Reverse Phase, 100 mm long, 5 µm particles 250 (2) 200 Area (1) -2256 Area (2)=9536 Area (3)=25638 150 N 100 50 ^ 0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5 6 6.5 7 7.5 8 8.5 9 9.5 10 Retention Time (min) Signal (arb, units) Signal (arb. units) 0
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