Suppose that you have an enzyme concentration at the molarity calculated in question 2 and the product that you monitor has a value of ε = 6200 M1 cm1. You ran eight reactions and found the following: Reaction # [substrate] (μM) Slope (Abs/min) 1 0 0.000 2 10 0.163 3 20 0.288 4 60 0.586 5 100 0.740 6 200 0.920 7 500 1.078 8 1000 1.143 M=4.45x10-M Calculate the values of Kcat and KM for the reaction (remember to include units). Show your plot and your calculations.

The Ksp for SrF2 is 2.45 x 10-9. Use this information to calculate the solubility of Strontium Fluoride in water (find the [Sr2+] and [F-] at equilibrium). SrF2 (s)  Sr2+ (aq) + 2F- (aq) I C E

The speed with which the protein bovine serum albumin (BSA) moves through water under the influence of an electric field was monitored at several values of pH, and the data are listed below. What is the isoelectric point of the protein?pH                           4.20     4.56     5.20       5.65       6.30        7.00Speed/(μm s-1)       0.50      0.18    -0.25     -0.65      -0.90      -1.25

A certain drug binds to a protein with Kd = 850.0 nM. What initial concentration of the drug would you need to add to a 50.0 µM solution of the protein so that 95.0% of the protein molecules are drug-bound at equilibrium? A. 1.62 μM B. 16.2 μΜ C. 47.5 μΜ D. 52.6 μM E. 63.7 μΜ

7. Copper(I) chloride salt is added to a solution of pure water to make a saturated solution. Upon dissolving, the concentration of copper(I) ion was found to be 4.15 x 10-4. What is the Ksp of copper (I) chloride? ©2022 F'22/S'23 Chemistry II A: 1.72 x 10-7 131

Construct the expression for Ksp for solid A9CN in aqueous solution. AGCN(s) = Ag*(aq) + CN-(aq) 1 Based on your knowledge of how the solid will dissociate in aqueous solution, use the tiles to form the expression. Ksp RESET [AGCN] 2[A9CN] [A9CNJ? [Ag*] 2[Ag*] [Ag*P [Ag*] 2[Ag*] [Ag*P [CN] 2[CN] [CN-P [CN²] 2[CN ] [CN-1?

5. Equilibrium Lab- Post Lab Question Name Marlysa Rackmyre #13 A student prepares two flasks. Flask A contained 5.00 mL of 3M HCl(aq) (density 1.05 g/mL). Flask B contained 5.00 mL of 3M HCl(aq) and 5.00 mL of ethyl acetate (density 0.893 g/mL).Flask A and Flask B are titrated against a standard solution of NaOH(aq) after Flask B has attained equilibrium. The titration values and the molarity of the sodium hydroxide for your titrations are shown in the data table that follows. Use the data to calculate Ke for the reaction shown below. EtAc + H₂O EtOH + HAC Titration reading Titration reading. molarity of No OH (aq) for flask A (mL) for flask B (ml) 2.737 5.28 12.42

The enzyme urease increases the rate of urea hydrolysis at pH 8.0pH 8.0 and 20 °C20 °C by a factor of 1014.1014. Suppose that a given quantity of urease can completely hydrolyze a given quantity of urea in 88 minutes at pH 8.0pH 8.0 and 20 °C.20 °C. How long would it take for this amount of urea to be hydrolyzed in the absence of urease at the same temperature and pH in sterile conditions? how many years?

7. A group of Colby students discovered a hydrolase that cleaves cellulose. After purifying the enzyme, an assay was performed by adding 0.1 μM of enzyme to the cellulose substrate concentrations shown below to characterize kinetic properties. [S] 2 x 10-6 M 4 x 10-6 1 x 10-5 2 x 10-5 4 x 10-5 1 x 10-4 2 x 10-3 1 x 10-² 4 x 10-² V (umol/min) 28 40 75 95 112 128 139 140 141 A. Use the data in the table above to estimate KM and Vmax for the enzyme. B. What is the keat of this enzyme? C. What is the catalytic efficiency of this enzyme?

8 Reactions between certain organic (alkyl) halides and water produce alcohols. Consider the overall reaction for t-butyl bromide (2-bromo-2-methylpropane). (CH3)3CBr(aq) + H2O(1) →(CH3)3COH(aq) + H*(aq) + Br "(ag) k[(CH3)3CB1]. The accepted mechanism for the The experimental rate law is rate = %3D reaction is shown below. (1) (CH3)3C-Br(aq) →(CH3)3C*(aq) + Br "(aq) [slow] (2) (CH3)3C*(aq) + H2O(1) →(CH3)3C-OH2*(aq) [fast] (3) (CH3)3C-OH2*(aq) →H*(aq) + (CH3)3C-OH(aq) [fast] a) Why doesn't H2O appear in the rate law? b) Write rate laws for the elementary steps. c) What intermediates appear in the mechanism? d) Show that the mechanism is consistent with the experimental rate law. ed to the of consumption of N