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What is the probable cause of the sample streaking across the TLC plate from origin to solvent front.
a. Too much sample
b. Too little sample
C. A developing solvent that is too polar
d. A developing solvent that is too non polar
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- 5. Summarize your conclusions from the TLC simulator. Circle the correct answer in each description about TLC separation of organic compounds on silica gel plates. a) A more polar compound has a lower/higher retention factor. b) A less polar compound has a lower/higher retention factor. c) A more polar solvent causes a lower/higher retention factor. d) A less polar solvent causes a lower/higher retention factor. e) To increase the retention factor of a spot, you should add more ethyl acetate/hexane.Match Column A with Column B. v Distance traveled by the solvent A. Silica gel G Adsorbent used in TLC B. Eluent v High affinity to stationary phase C. High Rf value v High affinity to mobile phase D. Point of origin v Liquid that comes out of the column E. Low Rf value F. Solvent front G. Silica gel H. Eluate13. Consider the TLC plate below to answer the question. What is the Rf of the top component? Note: the distance between the origin and the bottom spot is 1.4 cm. The distance between the two spots is 1.6 cm. Solvent front 7.2 8.4 1.6 Baseline
- What is the result of applying too much sample to the TLC plate? Select one: Some of the sample moves down instead of up Reduced separation between the spots O The sample moves past the solvent front O The spot does not leave the origin O The sample leeches out into the elution solvent2. In HPLC analysis, why Would peak area is preferred to peak height in evaluating the caffeine concentration? 3. Why was pure caffeine sample added into samples of unknown caffeine concentration in HPLC analysis?1. Define sparging. What is the significance of this step in an HPLC analysis? 2. What are the two types of pre-column? What is the purpose of each type? 3. What are the two types of packing used in HPLC column? Describe each type. Please answer numbers 1-3. Need asap
- What would happen to the retention time of a compound if the followingchanges were made?a. Decrease the flow rate of the carrier gasb. Increase the temperature of the columnc. Increase the length of the column8. Derivatiztion of sample is carried out to: a) Improve analyte peak shape analyte resolution b) Increase the detector response c) Improve d) All the aboveHow you can improve resolution in HPLC analysis
- In HPLC, why is purging or initialization being done before analysis of samples, explain.QUESTIONS D. How will the following affect the TLC separation? a) too much sample applied. b) Stationary phase is too active. c) Forgetting to remove the plate when the solvent has reached the of the plate. top d) Having too much solvent in the developing chamber so that its surface extends above the origin. e) Polarity of the solvent being too high. 2) Which dye would be more soluble in n-propyl alcohol, the blue or the yellow? How can you tell? 3) Which compound is more strongly adsorbed on silica, ortho- or para- nitroaniline? Correlate the R, values with the structures. 76Different from GCMS, why is an interface needed when coupling HPLC with MS?
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