This question is regarding the 6 areas that can be involved in the control of gen expression: Part 1 - Using the image below, indicate where the six major control points for eukaryotic gene expression are on the figure (Letters A - F). Part 2 - Please give a brief description of what is occurring at each point or the specific molecule(s) for letters A - F.

Compare and contrast (that means tell me how the are similar AND how they are different) gene regulation mechanisms in prokaryotes and eukaryotes. explain why regulation is more complex in multicellular eukaryotic organisms as compared to prokaryotic organisms by providing examples of eukaryotic gene regulation mechanisms (you don’t have to give me all the steps, just the concepts).

Based on the partial diploid sketched shown in Figure 3, where cross-hatched rectangles and the red bar indicate mutations that inactivate that particular genetic element of the operon, state if expression of LacO (Lac Operator) and LacY (Lac Permease) is (select one of the options below for each genetic element and briefly justify your answer): • constitutively ON (in the presence/absence of lactose) • constitutively OFF (in the presence/absence of lactose) • inducible by lactose • not enough information given to conclude • none of the above FIGURE 3: lact laco lacz- lacř lact laco lacz lac

What are the roles of histone deacetylation and histoneacetylation in gene regulation, respectively

Give the levels of Beta-galactosidase activity (high or low or zero) expected for the following partial diploids for the lac operon strains.

The extracellular protein factor Decapentaplegic(Dpp) is critical for proper wing development in Drosoph-ila (Figure Q21–3A). It is normally expressed in a narrowstripe in the middle of the wing, along the anterior–pos-terior boundary. Flies that are defective for Dpp formstunted “wings” (Figure Q21–3B). If an additional copyof the gene is placed under control of a promoter that isactive in the anterior part of the wing, or in the posterior part of the wing, a large mass of wing tissue composed ofnormal-looking cells is produced at the site of Dpp expres-sion (Figure Q21–3C and D). Does Dpp stimulate cell divi-sion, cell growth, or both? How can you tell?

CD3 is a signaling protein that is typically found only in the plasma membrane of immune system T lymphocytes. CD3 is composed of several different polypeptides, including a gamma chain, CD3γ. Scientists analyzed the promoter of the CD3γ chain gene for regulatory sequences that might have positive or negative effects on expression of the gene. The scientists cloned fragments of the CD3γ gene that included the first transcribed nucleotides plus up to 789 nucleotides of upstream regulatory sequences into plasmids in which the gene for the firefly enzyme luciferase immediately follows the fragments. The plasmids were then introduced into a line of T lymphocytes (Figure 1), and the cells were allowed to grow for a short while. Because the regulatory sequences of the CD3γ gene immediately precede the luciferase gene in the plasmids, the activity, either positive or negative, of the regulatory sequences affected the amount of luciferase gene expression by the T lymphocytes. Luciferase…