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MATLAB: An Introduction with Applications
6th Edition
ISBN: 9781119256830
Author: Amos Gilat
Publisher: John Wiley & Sons Inc
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[3] Instructions: Read each excerpt from a research paper and identify the dependent, independent, controlled variable (at least 1), experimental group/treatment, and control group.
Independent variable: | |
Dependent variable: | |
Controlled variable: | |
Experimental Group: | |
Control group: |

Transcribed Image Text:3. Antibacterial Assay
The test solution of each extract was prepared by dissolving 0.1g of the plant
extract separately. 1.0cm3 of dimethyl sulphoxide (DMSO) to get a concentration
of 100mg/cm3. The antibacterial activity was performed by filter paper disc
diffusion technique. Filter paper disc (Whatman No 1.6 mm diameter) were placed
in glass Petri dish and sterilized in a hot air oven. Iwu et al 2018b, the media (10g
nutrient Agar in 200cm3 distilled water, autoclaved at 115°C for 30 minutes) was
cooled to 50°C. The sterile nutrient Agar media were poured into the sterile Petri
dish and allowed to solidify. The bacteria were swabbed with a sterile wire loop.
Each disc was impregnated with 0.2cm3 of plant extract. Standard antibiotic
Ciprofloxacin was used as a control on a disc with DMSO 100mg/cm3. The discs
were used after drying them in an incubator 40°C to remove any trace of solvent.
Discs were introduced into the surface of the medium. The plates were microbated
at 37°C for 24 hours to obtain zones of inhibition. The experiments were repeated
three times for each extract and twice for reference antibiotics to minimize error
and the average of these values was recorded."
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