The molecular weight of hemoglobin is 64,500 daltons (= g mol-1). A. How much oxygen can be bound per g of hemoglobin? Express the values as mL per g of hemoglobin, where mL is the volume of O2. R =0.082 L atm mol-1 oK-1, oK =273 + oC. The molar volume of O2 is given as V = nRT/P where P = 1 atm and n= 1 mol. (Hint: 1.0 mol x 0.082 L atm mol-1 oK-1 x 273.2 oK / 1 atm = 22.4 L mol-1) (Hint: The number of moles of O2 that can be bound per g of hemoglobin is 4 x the moles of Hb in 1 g). B. The blood in a person contains 15 g of hemoglobin per 100 ml blood. How much oxygen does the blood contain at 95 mm Hg if hemoglobin is 98% saturated and the solubility of O2 is 0.003 mL dL-1 mmHg-1? (Hint: Total O2 content = HbO2 + free dissolved O2) C. Suppose the venous PO2 is 40 mm Hg, and that hemoglobin is 75% saturated at this point. What is the oxygen content of venous blood?

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The molecular weight of hemoglobin is 64,500 daltons (= g mol-1). A. How much oxygen can be bound per g of hemoglobin? Express the values as mL per g of hemoglobin, where mL is the volume of O2. R =0.082 L atm mol-1 oK-1, oK =273 + oC. The molar volume of O2 is given as V = nRT/P where P = 1 atm and n= 1 mol. (Hint: 1.0 mol x 0.082 L atm mol-1 oK-1 x 273.2 oK / 1 atm = 22.4 L mol-1) (Hint: The number of moles of O2 that can be bound per g of hemoglobin is 4 x the moles of Hb in 1 g). B. The blood in a person contains 15 g of hemoglobin per 100 ml blood. How much oxygen does the blood contain at 95 mm Hg if hemoglobin is 98% saturated and the solubility of O2 is 0.003 mL dL-1 mmHg-1? (Hint: Total O2 content = HbO2 + free dissolved O2) C. Suppose the venous PO2 is 40 mm Hg, and that hemoglobin is 75% saturated at this point. What is the oxygen content of venous blood? D. Suppose the cardiac output is 5 L min-1. Calculate the oxygen delivery to the tissues from B and C. (Hint: Rate of O2 transport = cardiac output * (arterial O2 content – venous O2 content)
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