The man's genetic defect appears to be due to the mutation of the promoter region of a protein X. This protein is an activator of an enzyme Y that detoxifies reactive oxygen species and free radicals. DNA sequence analysis using the Sanger method of the mutated region around the TATA box detected the following products: Normal With Genetic Disorder ddNTP +ddTTP tetranucleotide tetranucleotide pentanucleotide pentanucleotide heptanucleotide heptanucleotide +ddATP mononucleotide mononucleotide trinucleotide trinucleotide hexanucleotide +ddCTP dinucleotide dinucleotide nonanucleotide hexanucleotide nonanucleotide +ddGTP octanucleotide octanucleotide decanucleotide decanucleotide What base was altered and to what was it changed?

List two possible missense mutation effects on the new polypeptide.

A lilP mutant called lilPXS is isolated that produces a truncated polypeptide of only 6 AA in length. Describe a single basepair DNA change that would lead to this truncated version of the protein. Multiple options are possible（100 words maximum）

There are five substitution mutations in the dark-colored mutant Mc1r gene. Compare the DNA sequence of the light-colored wild-type Mc1r gene with the DNA sequence of the dark-colored mutant Mc1r gene. Indicate the locations of the five mutations by changing the font color to YELLOW for the five single DNA nucleotides that are mutated in the mutant Mc1r gene table. Using the information in the introduction, determine whether each of these mutations is a silent, missense, or nonsense mutation. Using the mutant Mc1r gene data, fill in the columns (including DNA, mRNA, and amino acid) in gene table 2 that contain a silent mutation with BLUE. Likewise, fill in the columns that contain a missense mutation with RED. Shade any columns that contain nonsense mutations with GREEN. Then  Of the five mutations you identified in the mutant Mc1r gene, how many are:                  substitutions           insertions              deletions (Enter a number on each line.) 2. Of the five mutations…