Chemistry
Chemistry
10th Edition
ISBN: 9781305957404
Author: Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher: Cengage Learning
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The chromatogram in the figure is of a mixture of 2 compounds,
carried out in a packed liquid chromatographic column of 25 cm
length. The flow rate was 0.40 mL min ¹. A species that is not retained
passes through the column in 5 minutes.
a) Determine the retention factors for the two components.
b) Calculate the resolution of the two peaks.
c) Find the average height of the plate.
d) What column length would be needed to achieve a resolution of
1.75?
Detector response
0
5.0
10
22.0
20
30.0
30
Time, min
38.0
40
39.0
50
50.0
60
62.0
70
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Transcribed Image Text:The chromatogram in the figure is of a mixture of 2 compounds, carried out in a packed liquid chromatographic column of 25 cm length. The flow rate was 0.40 mL min ¹. A species that is not retained passes through the column in 5 minutes. a) Determine the retention factors for the two components. b) Calculate the resolution of the two peaks. c) Find the average height of the plate. d) What column length would be needed to achieve a resolution of 1.75? Detector response 0 5.0 10 22.0 20 30.0 30 Time, min 38.0 40 39.0 50 50.0 60 62.0 70
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