Target of doublestranded rna during RNAi is determined by...... size   molecular weight  sequence   fragmentation

Describe in detail the process of RNAi including the major proteins involved. What is the role of RISC versus RITS? How is RISC versus RITS? How is siRNA used in biotechnology/ What are some limitations of RNAi?

To become functional, newly synthesized RNA must undergo a series of maturation steps such as excising unwanted intervening sequences and some modifications. Briefly describe this process.

Name the four classes of RNA and briefly explain their functional significance. In what reaction(s) do they participate?

2. H. Gobind Khorana deciphered the genetic code by (1) chemically synthesizing DNA molecules of known sequence, (2) using this synthetic DNA as a template to produce RNA in a reaction involving RNA polymerase and dNTPs, then finally (3) using this RNA in an in vitro translation experiment with the ribosome and amino acyl-tRNA (aa-tRNA) building blocks to produce peptides. For each single-stranded deoxyribonucleotide sequence Khorana synthesized (shown below), a single polypeptide was produced in the in vitro translation step (3): (1) Synthetic DNA d(TTT)n d(CCC)n d(AAA)n d(GGG)n d(AAACCC)n d(AC)n d(ACA)n a. (2) RNA encoded by synthetic DNA (3) Peptide product composition polylysine polyglycine polyphenylalanine polyproline Equal F and G Equal C and V polycysteine Fill in the RNA sequences that were produced by RNA polymerase in column (2). b. When Khorana conducted this work, one looming question in the field was whether or not the genetic code is overlapping or non-overlapping. In a…

what is the difference between leader mRNA and attenuator sequence? Please explain the function of attenuator sequence in attentuation

1)    Considering how readily RNA folds to form a secondary structure, why isn’t it used to store genetic information rather than double-stranded DNA? Cite multiple reasons. 2)  Do miRNA and siRNA both derive from the same source? Explain.

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IX. Dibuje la estructura del polinucleótido que aparece a continuación el cual corresponde a un codón en el mRNA que codifica para el aminoácido prolina. (Solamente se pide el dibujo de la estructura siguiendo el modelo de polinucleótido mostrado en clase). e). .) 5'-C-C-C-3' Show Transcribed Text IX. Draw the structure of the polynucleotide below which corresponds to a codon in the mRNA that codes for the amino acid proline. (Only a drawing of the structure following the polynucleotide model is requested)

Explain why each of the criteria listed below are important in selection of a guide RNA (hint: look under Instructions on CHOPCHOP).  Under what conditions do these criteria decrease the efficiency of binding of guide RNA to its target sequence (e.g. does more, or less, self-complementarity decrease efficiency)? a) % GC (= # of G and C’s in the guide RNA sequence divided by 20 – the length of the guide RNA; this may also be called GC content) b) Self-complementarity: c) Off-Targets (0, 1, 2, 3). In your answer, explain what the numbers 0, 1, 2, 3 indicate.  For example - Is it better to have off-target sites or no off-target sites? Are off-targets in the 1 column more or less likely to decrease efficiency than off-targets in the 3 column?