Table 1. Objective Lens Scanner Low Power Objective High Power Objective Oil Immersion Objective Objective magnification Ocular magnification Total Magnification
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- Comparison of the different objectives of the compound microscope Point of Comparison Scanner LPO НРО O1O Degree of magnification (X) 4х 10x 40x 100x Details of the specimen (least to most detailed) Area of field of view (smallest to biggest) Brightness of image (dimmest to brightest ) Working distance (mm)Complete Table 1 below by supplying the characteristics of each objective. Table 1. Numerical characteristics of microscope objectives. LPO НРО OIO Focal length (mm) Working distance (mm) Linear magnification (X) Numerical aperture (N.A..) Features (color of the band, diameter of the opening of the lens, etc.)II.VIRTUAL MICOSCOPE 4X 4X SPECIMEN 10X 10X 40X 40X 100X 100x COARS FOCUA TME FOOR LCHT AVAN 100X MAGNIFICATIO | 4X N USED IN THE VIRTUAL MICROSCOPE OCULAR MAGNIFICATIO (10X) TOTAL MAGNIFICATIO
- identify these parts of the microscope I have submitted these answers but there were 4 wrong 1.Ocular lens2.Diopter Adjustment3.Nose piece4.Stage5.Condenser6.Iris diaphragm7.Illuminator8.Course objective knob9.Fine adjustment knob10.Objective lensMatch each item with the correct compound light microscope part below. objective lenses fine adjustment knob diaphragm 1. choice D 2. choice K 3. choice GThe objective lenses of the microscopes used in this course are all parfocal. What does having parfocal objective lenses mean? Question options: The slide has to be moved away from the objective lens (i.e., lowered) when switching between objective lenses. None of these choices The objective lenses have optimized light gathering capability. Once in focus, you may switch between objective lenses with only fine focus adjustment required.
- Complete the following table by calculating the total magnification of the different objectives of the microscope with the given eyepieces. OBJECTIVES OCULAR Designation Magnification Focal Length Numerical Aperture (NA) Final magnification with: 10x 15x 25x Scanner 4x 46mm 0.10 LPO 10x 16mm 0.25 HPO 40x 4mm 0.55 OIO 100x 1.8mm 1.30Write T (True) or F (False) for the following statements. 1. The objective lens is the one nearest to the observer's eye. 2. Only special lens paper should be used to clean the objectives, oculars, and the condenser of the microscope. 3. The working distance is the distance between the specimen and the objective lens. _4. Magnifying a blurred image generally reveals further details. 5. When a microscope is parfocal, you should not have to use the coarse adjustment at higher magnification. _6. The total magnification of the object seen at high dry power is approximately 40X. 7. Immersion oil can be used to increase resolution of all the objective lenses in a brightfield microscope. _8. Always be sure to oil your microscope lenses before returning the instrument to its designated space. 9. Animals will not survive without mitochondria because they will not be able to make ATP. 10. The use of normal saline solution (NSS) in viewing cheek cells is to prevent lysis or crenation of cells.Answer these questions by watching the YouTube videos and reviewing the Powerpoints from Lab #4. 1. What is Refraction of light? 2. What is the difference between the Ocular lens and the Objective lens? 3. What is the purpose of the Revolving Nosepiece? 4. What is the difference between the Course Adjustment Knob and the Fine Adjustment Knob? 5. How do you calculate Total Magnification? 6. What is Resolution in terms of Microscopy? 7. What is the purpose of Oil Immersion? 8. What is the Diffraction Barrier and why does it exist? 9. What is the purpose of using stains and fluorescent dyes in microscopy? 10. What is the advantage of using an Electron Microscope? 11. What Objective lens should you always start with? 12. What is the purpose of the Iris Diaphragm on the Condenser? 13. How do you know your Objective lens has been adjusted properly? 14. Why should you not use Kimwipes…
- What part of the microscope is used to make small-scale adjustments to the height of the stage? Group of answer choices 1. fine focus 2. iris diaphragm 3. eye pieces 4. coarse focus 5. slide holder adjustmentThe significance of using immersion oil when using 1OOX objective lens is The significance of using immersion oll when uskng 100X objective lens is: It will increase the contrast by having same refractive index as the lens, loss of light by refraction, reflection and diffraction is minimized. the specimens need not be killed, fixed and stained more light is captured for better illumination, allowing the specimen to fluoresce i. il. iii. iv. A light microscope should have the following features contrast Resolution magnification fluorescence ii. iv.For a microscope that has an objective lens magnification of 100X and an ocular lens magnification of 5X, calculate the total magnification. 500X O 1000X O 50X O 100X O No listed answer is correct.