Suppose you are viewing a Gram-stained field of red rods and purple cocci through the microscope. What do you conclude?

Suppose you performed a Gram stain from a pure culture of bacteria and observed a field of red and purple bacilli. Adjacent cells were not always the same color. Provide an explanation for this observation.

why does are commonly used to stain cytoplasm granules blue

A student missed the laboratory period where the use of the microscope was demonstrated. The instructor asked the student to read the description in the laboratory manual and then proceed to examine bacterial cells with the oil immersion lens. The student skimmed the directions and began. After about 15 minutes of struggling, the student gave up in despair without seeing anything. Below is a detailed description of what the student did. How many mistakes did the student make and why didn’t the student see anything? a. Plugged in the microscope and turned the light source to maximum intensity. Made a wet mount and placed it on the stage with the low-power objective lens in position. Tried to focus with the coarse adjustment, but decided the bacteria were too small and needed to be seen with the high-power objective lens. Rotated the high-power objective lens into position, but saw the lens would likely touch the slide, so lowered the stage so that the objective lens rotated freely.…

Imagine you accidentally applied water during the decolorization step instead of ethyl alcohol. Which of the following outcomes would you expect? Both Gram-positive and Gram-negative bacteria would appear reddish-pink Both Gram-positive and Gram-negative bacteria would appear purple Gram-positive bacteria would appear purple and Gram-negative bacteria reddish-pink Gram-positive bacteria would appear reddish-pink and Gram-negative bacteria purple Gram-positive bacteria would appear colorless and Gram-negative bacteria reddish-pink

You are trying to look at a specimen with a compound microscope using the 4x objective, but it is very dark. Mention three parts of the microscope you could adjust that would help see your specimen in this darkness. Explain how you would adjust those parts.

Suppose that you are viewing a Gram-stained field of red rods and purple cocci through the microscope. What can you conclude about your culture?

Describe how the images obtained using a confocal microscope would appear different to those obtained using a widefield microscope.