short term habituation is due to changes in the presyna

In the discussion section the authors wrote “In this study, we observed that different paradigms of tES application could ameliorate motor function impairments in ICH induced by collagenase administration into the striatum of rats.”  a. Is the information in this quote about afferent of efferent?             b.By what word or words do you know that your answer to the question is correct?            c. Why did you choose this word or words as an answer to the question?

Link:     https://doi.org/10.1186/s12868-022-00692-1  A. In the effect of mitoxantrone on histopathological changes in the brain section of the results section the authors wrote “In Fig. 4, the demyelination of (A-C) the cerebellum and (F-H) brain stem was evaluated using LFB staining and median scored 2.5 in both sedentary and exercised untreated EAE rats indicating extensive demyelination.”  Why was demyelination evaluated in this study?   B. In the effect of mitoxantrone on histopathological changes in the brain section of the results section the authors wrote “Post-administration of mitoxantrone to sedentary and exercised groups smaller patches of demyelination with microcyst formation and lymphocytic infiltrates were seen; and bare unmyelinated axons were fewer (Figs. 3D, E, I, J and 4D, E, I, J).”  Based on this quote, what is mitoxantrone doing exactly/directly?

Which experimental technique would be most helpful in mapping the function of the cells in the primary motor cortex?     Select one: a. electrical stimulation b. cortical lesions c. Golgi stain d. intracellular recording  e. immunohstochemistry/immunocytochemistry