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SDS-PAGE w/o B-mercaptoethanol LANE 1 D LANE 2 C SDS-PAGE w/ B-mercaptoethanol LANE 1 LANE 2 B C D

SDS-PAGE w/o B-mercaptoethanol LANE 1 D LANE 2 C SDS-PAGE w/ B-mercaptoethanol LANE 1 LANE 2 B C D

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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D M SDS-PAGE w/o ß-mercaptoethanol LANE 1 D | || LANE 2 SDS-PAGE w/ P-mercaptoethanol LANE 1 D ||| LANE 2 Figure D.2. Electrophoretogram obtained after SDS-PAGE of the purified protein extract from redcrest and padisarah.
Transcribed Image Text:D M SDS-PAGE w/o ß-mercaptoethanol LANE 1 D | || LANE 2 SDS-PAGE w/ P-mercaptoethanol LANE 1 D ||| LANE 2 Figure D.2. Electrophoretogram obtained after SDS-PAGE of the purified protein extract from redcrest and padisarah.
3. Tighnari forgot to note in which sample lane he loaded the redcrest and padisarah purified protein extracts, however, previous studies show that one of the isolated major proteins from redcrest has 4 subunits of which 2 are specifically linked by disulfide bridges. Based on Figure D.2., in which lane did Tighnari load the purified protein extract from redcrest? Support your answer using the completed table below. Hint: Take note of the color intensity of the bands. LANE No. Protein Component Lane 1 Subunits based on SDS-PAGE w/o B- " mercaptoethanol Subunits based on SDS-PAGE w/ B-mercaptoethanol Observations (e.g., no. of subunits, subunits linked by disulfide bridges, etc.) Protein 1 Protein 2 Protein 1 Lane 2 Protein 2 *Note: for "subunits", kindly use the labels or identities based on Figure D.2. and Table D.1. (e.g. A, B, C, and etc.). 4. Which between the two major proteins (COL and LEI) is described on the available literature studies? Justify.
Transcribed Image Text:3. Tighnari forgot to note in which sample lane he loaded the redcrest and padisarah purified protein extracts, however, previous studies show that one of the isolated major proteins from redcrest has 4 subunits of which 2 are specifically linked by disulfide bridges. Based on Figure D.2., in which lane did Tighnari load the purified protein extract from redcrest? Support your answer using the completed table below. Hint: Take note of the color intensity of the bands. LANE No. Protein Component Lane 1 Subunits based on SDS-PAGE w/o B- " mercaptoethanol Subunits based on SDS-PAGE w/ B-mercaptoethanol Observations (e.g., no. of subunits, subunits linked by disulfide bridges, etc.) Protein 1 Protein 2 Protein 1 Lane 2 Protein 2 *Note: for "subunits", kindly use the labels or identities based on Figure D.2. and Table D.1. (e.g. A, B, C, and etc.). 4. Which between the two major proteins (COL and LEI) is described on the available literature studies? Justify.
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