DMSDS-PAGE w/o ß-mercaptoethanolLANE 1D| ||LANE 2SDS-PAGE w/ P-mercaptoethanolLANE 1D|||LANE 2Figure D.2. Electrophoretogram obtained after SDS-PAGE of the purified proteinextract from redcrest and padisarah. 3. Tighnari forgot to note in which sample lane he loaded the redcrest and padisarah purified proteinextracts, however, previous studies show that one of the isolated major proteins from redcrest has 4subunits of which 2 are specifically linked by disulfide bridges. Based on Figure D.2., in which lane didTighnari load the purified protein extract from redcrest? Support your answer using the completed tablebelow. Hint: Take note of the color intensity of the bands.LANENo.ProteinComponentLane 1Subunits based onSDS-PAGE w/oB-"mercaptoethanolSubunits based onSDS-PAGE w/B-mercaptoethanolObservations(e.g., no. of subunits,subunits linked by disulfidebridges, etc.)Protein 1Protein 2Protein 1Lane 2Protein 2*Note: for "subunits", kindly use the labels or identities based on Figure D.2. and Table D.1. (e.g. A, B, C,and etc.).4. Which between the two major proteins (COL and LEI) is described on the available literaturestudies? Justify.

Electrophoresis is defined as the migration of charged particles under the influence of an electric…

Answered: SDS-PAGE w/o B-mercaptoethanol LANE 1 D…

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Biology

SDS-PAGE w/o B-mercaptoethanol LANE 1 D LANE 2 C SDS-PAGE w/ B-mercaptoethanol LANE 1 LANE 2 B C D

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter16: Adult And Pediatric Dosages Based On Body Surface Area

Section: Chapter Questions

Problem 7.2P

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Question

D
M
SDS-PAGE w/o ß-mercaptoethanol
LANE 1
D
| ||
LANE 2
SDS-PAGE w/ P-mercaptoethanol
LANE 1
D
|||
LANE 2
Figure D.2. Electrophoretogram obtained after SDS-PAGE of the purified protein
extract from redcrest and padisarah.

3. Tighnari forgot to note in which sample lane he loaded the redcrest and padisarah purified protein
extracts, however, previous studies show that one of the isolated major proteins from redcrest has 4
subunits of which 2 are specifically linked by disulfide bridges. Based on Figure D.2., in which lane did
Tighnari load the purified protein extract from redcrest? Support your answer using the completed table
below. Hint: Take note of the color intensity of the bands.
LANE
No.
Protein
Component
Lane 1
Subunits based on
SDS-PAGE w/o
B-
"
mercaptoethanol
Subunits based on
SDS-PAGE w/
B-mercaptoethanol
Observations
(e.g., no. of subunits,
subunits linked by disulfide
bridges, etc.)
Protein 1
Protein 2
Protein 1
Lane 2
Protein 2
*Note: for "subunits", kindly use the labels or identities based on Figure D.2. and Table D.1. (e.g. A, B, C,
and etc.).
4. Which between the two major proteins (COL and LEI) is described on the available literature
studies? Justify.

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