Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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27) SDS-PAGE
28) Reading DNA, RNA and protein sequences
29) Cloning vectors
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- create a concept map illustrating the relationships among these key terms: 1. Foreign DNA; 2. Restriction endonuclease; 3. Ligase; 4. Plasmid; 5. Origin of replication; 6. Recombinant Plasmid;7. Multiple cloning sites.arrow_forward39. Which of the following are general features found in a plasmid used for bacterial cloning? Group of answer choices Origin of replication Multiple cloning site Multiple cloning site and antibiotic resistance gene Multiple cloning site, antibiotic resistance gene, and origin of replication Locus of transformation Locus of transformation, multiple cloning site, and antibiotic resistance gene Antibiotic resistance genearrow_forwardplease see attachedarrow_forward
- How would you go about cloning this amplified DNA into pL4440?Using your knowledge of cloning describe 5 important aspects of the method in detailsarrow_forwardList and explain the steps of DNA cloning using pUC8.arrow_forwardTwo techniques used to make large amounts of a segment of DNA or gene of interest are: polymerase chain reaction and DNA cloning RNA interference and DNA sequencing polymerase chain reaction and DNA sequencing polymerase chain reaction and RNA interference RNA interference and CRISPRarrow_forward
- A recombinant plasmid can be used to a.) prevent nondisjunction at meiosis. b.) double the number of chromosomes in a plant cell. c.) cut DNA into restriction fragments. d.) transform a bacteriumarrow_forwarda. What is the purpose of molecular cloning?b. What purpose do selectable markers serve in vectors?c. What is the purpose of the origin of replication in aplasmid vector?d. Why do cloning vectors have polylinkers?arrow_forwardPart 1. Gene Cloning 1) How is a gene inserted into a plasmid cloning vector? Be specific, but be brief. No more than 2 (non-run-on) sentences please, and do not exceed this space. 2) A geneticist has transformed a vector with an ampicillin resistance gene into E. coli cells and plated those cells on media containing ampicillin. She comes back 24 hours later and sees only blue colonies on the media. a) How does she know that the cells growing on the media were successfully transformed with the vector? b) What does it mean that the colonies are blue?arrow_forward
- please explain this picturearrow_forward2) Transgenic animal refers to an organism whose genome has been altered by the transfer of a gene or genes from another species or breed. Cows, pigs and goats had been modified especially for pharmaceutical purposes. Explain three (3) most used transfection technology for the creation of transgenic animals.arrow_forwardQ4) What are the 3 main components required for cloning? Q5) What is 16S rDNA, and why would cloning it be useful? Q6) How big is the 16S rDNA ‘amplicon’ we would be cloning?arrow_forward
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