[Review Topics](References)The isoelectric point, pl, of the protein lipase A is 4.9 , while that of nitrate reductase is 4.2 .What is the net charge of lipase A at pH 5.1 ?What is the net charge of nitrate reductase at pH 2.5 ?The isoelectric point of glycine is 5.97 ; arginine, 10.76.During paper electrophoresis at pH 7.1 , toward which electrode does glycine migrate?During paper electrophoresis at pH 7.5 , toward which electrode does arginine migrate? Revlew Topics]References)The isoelectric point, pl, of the protein 3a hydroxysteroid dehydrogenase is 6.1, while that of chymotrypsinogen A is 9.1What is the net charge of 3a hydroxysteroid dehydrogenase at pH 5.1What is the net charge of chymotrypsinogen A at pH 8.5 7The isoelectric point of histidine is 7.59 ; threonine, 5.6.During paper electrophoresis at pH 7.1, toward which electrode does histidine migrate?During paper electrophoresis at pH 4.5 , toward which electrode does threonine migrate?

At isoelectric point is the point where the net charge on protein becomes zero.

Review Topic [References) The isoelectric point, pl, of the protein lipase A is 4.9 , while that of nitrate reductase is 4.2. What is the net charge of lipase A at pH 5.1 ? What is the net charge of nitrate reductase at pH 2.5 The isoelectric point of glycine is 5.97 ; arginine, 10.76. During paper electrophoresis at pH 7.1 , toward which electrode does glycine migrate? During paper electrophoresis at pH 7.5 , toward which electrode does arginine migrate?

Review Topic [References) The isoelectric point, pl, of the protein lipase A is 4.9 , while that of nitrate reductase is 4.2. What is the net charge of lipase A at pH 5.1 ? What is the net charge of nitrate reductase at pH 2.5 The isoelectric point of glycine is 5.97 ; arginine, 10.76. During paper electrophoresis at pH 7.1 , toward which electrode does glycine migrate? During paper electrophoresis at pH 7.5 , toward which electrode does arginine migrate?

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Chemistry Given TETRAPEPTIDE: Prolinyl-Argininyl-Cysteinyl-Tryptophan Predict the direction of the peptide in electrophoresis at pH 7 and pH11.5. (Use table on the upper right for the estimated pK values of some functional groups).
The isoelectric point (pl) can best be calculated by: Averaging the sums of all pkas. Averaging the sum of the two pKas closest to physiological pH. Averaging the two pKas "sandwiching" the zwitterionic form. Averaging the two pKas of the alpha-amino and alpha-carboxy groups.
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An alpha carboxylic acid group (COOH) of protein amino acids has a pka = 2.4. The ratio of %3D dissociated versus undissociated molecule (CO0-/COOH) at pH = %3D 2.4 will be
Please don't provide handwritten solution .
Peptides can be separated using an ion-exchange column based on their isoelectric (pl) values. At which pH values would two different peptides, one with a pl of 5.6 and the other with a pl of 9.1, bind to a cation- and anion-exchange column? Each peptide may be capable of binding to each column at more than one pH value. anion-exchange column at pH = 3.1 pH = 7.2 pH = 10.6 ****** cation-exchange column at pH = 3.1 pH = 7.2 PH =10.6 Answer Bank peptide B pl = 9.1 peptide A pl = 5.6
QUESTION 4 The table below shows the pka values for the various functional groups on cysteine. In which of the following forms will cysteine predominantly be found at pH 9.1? group pKa carboxyl (-COOH) 1.96 sulfhydryl (-SH) 8.18 ammonium (-NH3*) 10.13 А. В. CH,- SH CH,- SH H,N- CH- COOH H,N- CH- COo* | С. D. CH,-S CH,-S H,N - CH COO H,N- CH-COO O A OB OD
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Answer both questions
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The isoelectric point, pl, of the protein soybean trypsin inhibitor is 4.5 , while that of clostripain is 4.8 . What is the net charge of soybean trypsin inhibitor at pH 5.1 ?[ What is the net charge of clostripain at pH 2.5 ?[ The isoelectric point of tyrosine is 5.66 ; tryptophan , 5.89. During paper electrophoresis at pH 4.5 , toward which electrode does tyrosine migrate? [ During paper electrophoresis at pH 6.5 , toward which electrode does tryptophan migrate?
Which of the following amino acids is most likely to have a positively-charged side group under normal physiological pH conditions? O a. Lysine O b. Alanine O c. Leucine O d. Glycine O e. Aspartic acid Next page Previous page Time left 1:33:55 Quiz navigation 271 PM 今 D 572 2022 DELL Delete PgUp PgDn F12 PrtScr Insert F9 F10 F11 F7 F8 F5 F6 F2 F3 F4 Esc F1 Backspace & % %3D @ # 8 6 7 3 1 Y Enter J K A s D F ck St