[Review Topics](References)The isoelectric point, pl, of the protein lipase A is 4.9 , while that of nitrate reductase is 4.2 .What is the net charge of lipase A at pH 5.1 ?What is the net charge of nitrate reductase at pH 2.5 ?The isoelectric point of glycine is 5.97 ; arginine, 10.76.During paper electrophoresis at pH 7.1 , toward which electrode does glycine migrate?During paper electrophoresis at pH 7.5 , toward which electrode does arginine migrate? Revlew Topics]References)The isoelectric point, pl, of the protein 3a hydroxysteroid dehydrogenase is 6.1, while that of chymotrypsinogen A is 9.1What is the net charge of 3a hydroxysteroid dehydrogenase at pH 5.1What is the net charge of chymotrypsinogen A at pH 8.5 7The isoelectric point of histidine is 7.59 ; threonine, 5.6.During paper electrophoresis at pH 7.1, toward which electrode does histidine migrate?During paper electrophoresis at pH 4.5 , toward which electrode does threonine migrate?

At isoelectric point is the point where the net charge on protein becomes zero.

Review Topic [References) The isoelectric point, pl, of the protein lipase A is 4.9 , while that of nitrate reductase is 4.2. What is the net charge of lipase A at pH 5.1 ? What is the net charge of nitrate reductase at pH 2.5 The isoelectric point of glycine is 5.97 ; arginine, 10.76. During paper electrophoresis at pH 7.1 , toward which electrode does glycine migrate? During paper electrophoresis at pH 7.5 , toward which electrode does arginine migrate?

Review Topic [References) The isoelectric point, pl, of the protein lipase A is 4.9 , while that of nitrate reductase is 4.2. What is the net charge of lipase A at pH 5.1 ? What is the net charge of nitrate reductase at pH 2.5 The isoelectric point of glycine is 5.97 ; arginine, 10.76. During paper electrophoresis at pH 7.1 , toward which electrode does glycine migrate? During paper electrophoresis at pH 7.5 , toward which electrode does arginine migrate?

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Chemistry Given TETRAPEPTIDE: Prolinyl-Argininyl-Cysteinyl-Tryptophan Predict the direction of the peptide in electrophoresis at pH 7 and pH11.5. (Use table on the upper right for the estimated pK values of some functional groups).
A mixture of three proteins with pl = 7.5, 6.5, and 5.5 is separated using a DEAE-Sepharose column by eluting (washing) with buffer and NaCl. The column is eluted with aqueous buffer with a pH of 8.0, and an increasing [NaCl]. Which of the following proteins will elute from the column last? A The protein with pl = 5.5 B The protein with pl = 6.5 ☑ The protein with pl = 7.5 D These three proteins will not be separated, and will elute together. Your answer
The biruet method uses what reaction to quantify total protein in serum? Question 4 options: A) Cupric ions + peptide bonds in alkaline medium --> violet colored chelate B) Ferric ions + carboxyl bonds in alkaline medium --> aqua colored chelate C) Cupric ions + carboxyl bonds in acidic medium --> aqua clored chelate D) Nitrogen determined via titration with HCl
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The isoelectric point (pl) can best be calculated by: Averaging the sums of all pkas. Averaging the sum of the two pKas closest to physiological pH. Averaging the two pKas "sandwiching" the zwitterionic form. Averaging the two pKas of the alpha-amino and alpha-carboxy groups.
Enz-SH + Ag* - Enz-S-Ag + H* The affinity of Ag* for sulfhydryl groups is so great that Ag* can be used to titrate -SH groups quantitatively. To 10.0 mL of a solution containing 3.5 mg/mL of a pure enzyme, an investigator added just enough AGNO, to completely inactivate the enzyme. A total of 0.536 umol AGNO, was required. Calculate the minimum molecular weight (M,) of the enzyme. minimum M, = The determined M, is not exact, but it is a minimum value for the M, of the enzyme. Why does the M, value obtained this way give only the minimum molecular weight? There is only one polypeptide chain. There are no disulfide bonds in the protein. There is only one titratable -SH group per protein molecule. The Ag* would also react with the terminal carboxyl group.
An alpha carboxylic acid group (COOH) of protein amino acids has a pka = 2.4. The ratio of %3D dissociated versus undissociated molecule (CO0-/COOH) at pH = %3D 2.4 will be
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QUESTION 4 The table below shows the pka values for the various functional groups on cysteine. In which of the following forms will cysteine predominantly be found at pH 9.1? group pKa carboxyl (-COOH) 1.96 sulfhydryl (-SH) 8.18 ammonium (-NH3*) 10.13 А. В. CH,- SH CH,- SH H,N- CH- COOH H,N- CH- COo* | С. D. CH,-S CH,-S H,N - CH COO H,N- CH-COO O A OB OD
The secondary structure of proteins results primarily from which of the following interactions? a. formation of disulfide linkages b. formation of hydrogen bonds c. formation of salt bridges d. more than one response is correct e. no correct response H2 + CH2 = CH2 --> CH3CH3Which of the following could be a substrate for an enzyme that has an induced-fit active site and catalyzes the reaction above?
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