Researchers studying the bacterium Escherichia coli split a population of the bacteria into two samples. Sample 1 was transformed with a plasmid containing a gene that makes the bacteria resistant to the antibiotic kanamycin. Sample 2 was transformed with a plasmid lacking the antibiotic resistance gene. A portion of each sample was then added to growth plates containing just nutrients or growth plates containing nutrients and kanamycin. After being allowed to grow for 24 hours at 37 0 C , the number of colonies on each growth plate was counted (Table 1). Table 1. BACTERIAL GROWTH FOLLOWING TRANSFORMATION WITH OR WITHOUT THE KANAMYCIN RESISTANCE GENE Place # Transforming plasmid Contains Kanamycin Resistance Gene Growth Media Results 1 No Nutrient Only Too may colonies to count (lawn) 2 yes Nutrient Only Too any colonies to count (lawn 3 No Nutrient with Antibiotic No colonies observed 4 Yes Nutrient with Antibiotic 7 colonies observed Which of the following claims is best supported by the data in Table 1? A. The transformation procedure killed all the bacteria that were added to plate 3. B. More bacteria on plates 1 and 2 were successfully transformed than on any other plate. C. None of the bacteria on plate 2 were successfully transformed with the kanamycin resistance gene D. Only the bacteria that were successfully transformed with the kanamycin resistance gene grew on plate
Bacterial Morphology
The bacteria are prokaryotic organisms that are single-celled, and are found to exist as free-living and possess a microscopic size. The morphology is found to vary in the bacteria, where some of them are identified as individual organisms and the others are detected as colonies. The size and shape of the bacterial cell also represent its morphology.
Bacterial cell structure
Bacteria are single-celled, tiny creatures that may enter healthy tissues and grow rapidly. Bacteria are microscopic organisms that are tiny and unicellular. These are members of the prokaryote kingdom. They live in water, air, soil, and all-natural environments. They are used in industrial and therapeutic processes, and they support a wide range of plant and animal life. The first organism to appear on the planet. Bacteria-like creatures are the oldest known fossils. Bacteria can consume a wide range of organic and inorganic elements, and some may even survive in harsh conditions.
Researchers studying the bacterium Escherichia coli split a population of the bacteria into two samples.
Sample 1 was transformed with a plasmid containing a gene that makes the bacteria resistant to the
antibiotic kanamycin. Sample 2 was transformed with a plasmid lacking the antibiotic resistance gene.
A portion of each sample was then added to growth plates containing just nutrients or growth plates
containing nutrients and kanamycin.
After being allowed to grow for 24 hours at 37 0 C , the number of colonies on each growth plate was
counted (Table 1).
Table 1. BACTERIAL GROWTH FOLLOWING TRANSFORMATION WITH OR WITHOUT THE
KANAMYCIN RESISTANCE GENE
Place # Transforming plasmid
Contains Kanamycin
Resistance Gene
Growth Media Results
1 No Nutrient Only Too may colonies to count (lawn)
2 yes Nutrient Only Too any colonies to count (lawn
3 No Nutrient with Antibiotic No colonies observed
4 Yes Nutrient with Antibiotic 7 colonies observed
Which of the following claims is best supported by the data in Table 1?
A. The transformation procedure killed all the bacteria that were added to plate 3.
B. More bacteria on plates 1 and 2 were successfully transformed than on any other plate.
C. None of the bacteria on plate 2 were successfully transformed with the kanamycin resistance gene
D. Only the bacteria that were successfully transformed with the kanamycin resistance gene grew on plate
4.
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