Researchers studying the bacterium Escherichia coli split a population of the bacteria into two samples. Sample 1 was transformed with a plasmid containing a gene that makes the bacteria resistant to the antibiotic kanamycin. Sample 2 was transformed with a plasmid lacking the antibiotic resistance gene. A portion of each sample was then added to growth plates containing just nutrients or growth plates containing nutrients and kanamycin. After being allowed to grow for 24 hours at 37 0 C , the number of colonies on each growth plate was counted (Table 1). Table 1. BACTERIAL GROWTH FOLLOWING TRANSFORMATION WITH OR WITHOUT THE KANAMYCIN RESISTANCE GENE Place # Transforming plasmid Contains Kanamycin Resistance Gene Growth Media Results 1 No Nutrient Only Too may colonies to count (lawn) 2 yes Nutrient Only Too any colonies to count (lawn 3 No Nutrient with Antibiotic No colonies observed 4 Yes Nutrient with Antibiotic 7 colonies observed Which of the following claims is best supported by the data in Table 1? A. The transformation procedure killed all the bacteria that were added to plate 3. B. More bacteria on plates 1 and 2 were successfully transformed than on any other plate. C. None of the bacteria on plate 2 were successfully transformed with the kanamycin resistance gene D. Only the bacteria that were successfully transformed with the kanamycin resistance gene grew on plate

Researchers studying the bacterium Escherichia coli split a population of the bacteria into two samples.
Sample 1 was transformed with a plasmid containing a gene that makes the bacteria resistant to the
antibiotic kanamycin. Sample 2 was transformed with a plasmid lacking the antibiotic resistance gene.
A portion of each sample was then added to growth plates containing just nutrients or growth plates
containing nutrients and kanamycin.
After being allowed to grow for 24 hours at 37 0 C , the number of colonies on each growth plate was
counted (Table 1).
Table 1. BACTERIAL GROWTH FOLLOWING TRANSFORMATION WITH OR WITHOUT THE
KANAMYCIN RESISTANCE GENE
Place # Transforming plasmid
Contains Kanamycin
Resistance Gene

Growth Media Results

1 No Nutrient Only Too may colonies to count (lawn)
2 yes Nutrient Only Too any colonies to count (lawn
3 No Nutrient with Antibiotic No colonies observed
4 Yes Nutrient with Antibiotic 7 colonies observed
Which of the following claims is best supported by the data in Table 1?
A. The transformation procedure killed all the bacteria that were added to plate 3.
B. More bacteria on plates 1 and 2 were successfully transformed than on any other plate.
C. None of the bacteria on plate 2 were successfully transformed with the kanamycin resistance gene
D. Only the bacteria that were successfully transformed with the kanamycin resistance gene grew on plate
4.