Question 3. The nutrient broth is a basic media used for growing a broad variety of microorganisms in the laboratory. The nutrient broth consists mainly of 1.5 gL1 beef extract, 3gl1 yeast extract and 5 gL1 sodium chloride dissolved in distilled water. The nutrient agar is prepared by adding the agar at the desired amount into nutrient broth. Answer the following questions. a) How would you prepare a 500 ml nutrient broth? b) How would you prepare 2% (w/v) nutrient agar for 1L? c) How would you prepare a 100 ml nutrient broth supplemented with 100µg/ml ampicillin antibiotic? The stock concentration for ampicillin solution is 100 mg/ml.

Question 3. The nutrient broth is a basic media used for growing a broad variety of microorganisms in the laboratory. The nutrient broth consists mainly of 1.5 gL1 beef extract, 3gl1 yeast extract and 5 gL1 sodium chloride dissolved in distilled water. The nutrient agar is prepared by adding the agar at the desired amount into nutrient broth. Answer the following questions. a) How would you prepare a 500 ml nutrient broth? b) How would you prepare 2% (w/v) nutrient agar for 1L? c) How would you prepare a 100 ml nutrient broth supplemented with 100µg/ml ampicillin antibiotic? The stock concentration for ampicillin solution is 100 mg/ml.

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter1: The Clinical Laboratory

Section1.7: The Metric System

Problem 1P

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