Question 3 [10] 3.1. (i) Describe the differences between a linear ToF mass spectrometer and an orthogonal ToF instrument. (ii) What are the advantages and limitations of these two geometries? (2) (3) (3) 3.2. Describe three ways of introducing a sample into an ICP torch. 3.3. A chromatogram of a mixture of compound X and Y provided the following data (see Table 3.1): Table 3.1: Chromatographic data for U and V 3 Retention time (min) Peak width (at base) Mobile phase peak 0.81 0.01 Compound X 9.35 Compound Y 10.76 1.13 1.19 (i) For compound X, calculate (correct to 3 sig figs) its retention factor. (1) (ii) Calculate (correct to 3 sig figs) the selectivity factor for compound X and Y. (1)

A gas chromatogram of a mixture of toluene and ethyl (a) Measure w1/2 for each peak to the nearest 0.1 mm. When the thickness of the pen trace is significant relative to the length being measured, it is important to take the pen width into account. It is best to measure from the edge of one trace to the corresponding edge of the other trace, as shown at the bottom of the left column. (b) Find the number of theoretical plates and the plate height for each peak.

1) Study the chromatograph (below) of a mixture of Compounds A and B, run on the GCs in the teaching labs at CU Boulder. Compound A has the shorter retention time. STAAT 61 1.11 227 RT TYPE AREA XXXX XXXX XXXX AREAS 0.009 55874 44.117 ARIHT 0.61 XX XX XX 1.11 2.27 XX XX XX TOTAL AREA=XX MUL FACTOR=XX 1. What is the retention time of compound A? Compound B? 2. Which compound is present in a larger amount? 3. Which compound has the lower boiling point? 4. What would happen to the retention times of compounds A and B if the column temperature were raised? 5. You suspect that compound B is octane. What can you do to provide supporting evidence for this hypothesis?

In a chromatographic separation of two components A and B. Component A has a higher Rf value than component B. This implies that: A. Component B has a higher affinity than A for the mobile phaseB. component B elutes first in the columnC. A has a higher affinity than B for the mobile phaseD. Component A has a higher affinity than B for the stationary phase.

1,3,5 what the correct answer?

Kk.312.

QUESTION T5 Why were the areas underneath the peaks in the gan chromatographs you eobtained mutiplied by a weight tactor? DA the corrction factor compensates for user error OB Rame lonization detectors (FID) respond slightly dfferent to compounds with diferent molecular weights OCnone of the answers OD. the GC (GOW MAC 350) used contains a quadrapole mass spec detector whose response must be corrected for the miz ratio of the diferent ions obtainen for diferert moleculestune OE not all molecules have the same themmal conductivity QUESTION 16 Which list of functional groups is present in the anti-viral drug Arbidol HO Br O A Thioether, alkene, ester, amide, amine O 8. Thioether alcohol, ester, amine OC Alcohol, ether, amide, epoxide, thioester OD. Ether, ketone olefin, amine, alcohol O E Thioalcohol, alkene, ether, amine, nitro

Don't use AI.

Directions: (1) Determine the total volume of the standards by using a formula in the appropriate boxes (your instructor will help you with this). (2) Determine the concentration of Cu2+ in each standard, also using a formula in the appropriate cells. (3) Plot the absorbance as a function of Cu2+ concentration. Determine the equation for the best-fit line. 1.0 M ethylenediamine (mL) concentration Cu2+ absorbance (AU) at 550 nm 0.01 M CuSO4 5H2O H2O total volume stock Cu2+ standard (mL) (mL) (mL) (units) concentration (M) 1 0.50 0.00 9.50 0.025 0.01 2 0.50 0.50 9.00 0.031 0.01 3 0.50 1.00 8.50 0.048 0.01 4 0.50 2.00 7.50 0.153 0.01 5 0.50 3.00 6.50 0.223 0.01 0.50 4.00 5.50 0.307 0.01 7 0.50 5.00 4.50 0.384 0.01

[1] In paper chromatography, why do some ions move only a short distance from the point of application while others move much more?