Question 29 Which of these is a correct statement about bacterial transformation? O Bacteria can take up any length of DNA by transformation. O Bacteria take up naked DNA from their surroundings during transformation. O All bacteria are naturally competent to undergo transformation under any condition. O A bacteria can only take up DNA from another living cell during transformation.

Question #3: CRISPR has been used to cure an individual from sickle cell. Below is a Sanger electropherogram of a sequence from a patient without sickle cell and one with sickle cell. Sequence from a normal individual mmmm Sequence from the diseased individual G T GIIC A GC A Se SCIENCEphe A G A SCIENCE SCIENCEphoto G a) Where is the change in the sequence and what is the consequence to the protein sequence of this mutation? b) Below is an image of the normal and diseased quaternary hemoglobin protein. What is different about the protein shape and why does that structure have a huge impact on its function (please name the function!)? Adult haemogBRAR G G G G A G Sickle Cell haemoglobin S Structure a s RARY COLIBRARY c) If you were to use CRISPR to modify the genome of a diseased individual, to which nucleotides might you design your guide RNA? Why? d) RNA Seq is used to determine off-target effects of Cas9 cleavage. Why is this an appropriate tool to determine these effects? e) Data on…

QUESTION 20 Which of the following polymerases is found in the nucleolus? O A. DNA polymerase III O B. RNA polymerase III O C. RNA polymerase I D. RNA polymerase II - O E. Both A and B

QUESTION 3 which of the following is true for methyl directed repair O A. methyl directed repair cannot distinguish the template strand from the newly replicated strand. O B. methyl directed repair changes both the template strand and the newly replicated strand. O C. methyl directed repair corrects the DNA strand that is methylated. O D. methyl directed repair corrects the mismatch by changing the newly replicated strand. O E. methyl directed repair corrects the mismatch by changing the template strand.