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Genetics Q2
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- Question 43 The addition of restriction endonucleases in the cloning process is done following the ligation with DNA ligase. A) True B) FalseQuestion 1: Define and give an example of the following: Codon, Start Codon, Anticodon, and Stop Codon. a) How is information from the DNA code copied and moved to the cytoplasm? b) What are the base pairing rules followed when one DNA strand is read and an mRNA made? Question 2: What eukaryotic sub-cellular structure is responsible for making proteins? a) Is this sub-cellular structure present in prokaryotes? b) What is the name of the enzyme responsible for making the mRNA? c) Is DNA always inside the nucleus of eukaryotic cells?How does the aminoacyl-tRNA synthetase that attaches the amino acid proline know which tRNA to attach the proline to?Question 24 options: A) the synthetase recognizes the sequence of the first 3 bases on the 3' end of the tRNA B) the synthetase reads the anticodon as well as a few other unique bases in tRNAPro C) proline only fits into the 3-dimensional structure of the tRNAPro D) the synthetase randomly chooses a tRNA
- Which of the following recognizes the mRNA codon 5' - U A A - 3'?Question 29 options: A) a special termination tRNA B) a special termination amino acid C) a special termination protein D) Kozak's sequence E) aminoacyl tRNA synthetaseQuestion 7 A unique 5'CCA terminal sequence is found in all TRNAS to be able to carry the correct amino acid to the ribosome. (A) True B) False Question 8 Predominant nucleotides during protein synthesis are the GTPS. A True B) False Question 9 The nucleoside monophosphates are seen in metabolic pathways because their phosphoric anhydride bonds can be hydrolyzed off for energy. (A True False Question 10 The cyclic form of the sugar in DNA is a beta-D-3-deoxyribofuranose. A True FalseQuestion 28 The anticodon of a tRNA is 5'UUG. What codon(s) can be theoretically recognized by this tRNA? A) 5'AAC & 5' GAC B) 5'CAA only C) 5'CAA & 5'CAG D 5'AAC only
- Question 1: Translate the mRNA codon AGC a) Transcribe the DNA sequence AGC TAC CAG into mRNA Question 2: Translate the DNA sequence AAT GAC CCG into an amino acid sequence, remembering to translate from the mRNA code. Not the DNA, not the tRNA! a) Describe one thing you learned from practicing transcription and translation.Question 24 In prokaryotes, the sigma factor recognizes base sequences in the which facilitates RNA polymerase binding and initiates RNA synthesis. A) enhancer B) regulatory sequence c) promoter D) operatorQuestion 1: What does the term "double helix" mean with regard to DNA? a) The genetic information to make a protein is contained in a gene. List the three parts of a gene given in the tutorial and a short description of each segments' function. b) What form does a strand of DNA need to be in so that it can be seen using a compound (light) microscope? Question 2: What does tRNA stand for? Describe the role tRNA has in protein synthesis. a) Describe what a peptide bond is using 1-2 complete sentences. b) Describe what happens to the newly formed protein chain next.
- What is Kozak sequence ? who invented this ?Transposons in bacterial genomes can carry genes,including those conferring ________ resistanceWhich of the following anticodons can recognize BOTH codons for Aspartate (Asp)?Question 36 options: A) 3'-CUG-5' B) 3'-GAC-5' C) 3'-GAU-5' D) 3'-CUk2C-5' E) 3'-CUC-5'
