QUESTION 2 A mixture of Alanine (pl 6.02), Arginine (pl 10.76), Aspartic Acid (pl 2.98), Histidine (pl 7 59) and Phenylalanine (pl 5 48) are separated by cation exchange chromatography What is the order of elution of these amino acids if you use gradient buffer system from pH 10 to pH 2 ? First 1. Alanine Second 2. Arginine Third 3, Aspartic Acid 4. Histidine Fourth 5. Phenylalanıne 6. No separation v Fifth

QUESTION 2 A mixture of Alanine (pl 6.02), Arginine (pl 10.76), Aspartic Acid (pl 2.98), Histidine (pl 7 59) and Phenylalanine (pl 5 48) are separated by cation exchange chromatography What is the order of elution of these amino acids if you use gradient buffer system from pH 10 to pH 2 ? First 1. Alanine Second 2. Arginine Third 3, Aspartic Acid 4. Histidine Fourth 5. Phenylalanıne 6. No separation v Fifth

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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The amino acid Asp is triprotic with pK₁ = 1.88, pK₂ = 9.60, and pK3 = 3.65. What is the charge of the a-NH3* functional group at pH 11? -1 What is the net charge of Asp at pH 11? [Select] = The amino acid Asp is triprotic with pK1: 1.88, pK2 = 9.60, and pK3 = 3.65.
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Determine the isoelectric point of amino acids lysine and glycine. Write the solution. Draw the isoelectric point graph of the titration of each amino acid just like the picture
pka = 2.20 HO НО pKa = 10.07 OH Incorrect 9.82 Incorrect 6.84 Correct: 10.07 Incorrect 3.86 Incorrect 9.95 "NH₂ pka = 3.86 pka = 9.82 In your lab you make the dipeptide Tyronsie-Aspartic Acid and now are studying the physical properties of a 0.40 M solution of your product. What will the pH of the solution be when the overall charge on the dipeptide is -2 1/2.
The isoelectric point (pl) can best be calculated by: Averaging the sums of all pkas. Averaging the sum of the two pKas closest to physiological pH. Averaging the two pKas "sandwiching" the zwitterionic form. Averaging the two pKas of the alpha-amino and alpha-carboxy groups.
I only need the column: NAME OF THE TEST and (+) RESULT
Question 1 of 3 Why do amino acids not undergo condensation reactions to form peptide bonds in aqueous solution? Condensation would not produce a peptide bond in any solvent There is no pH at which the carboxylic acid remains in the acid form There is no pH at which the amino group remains in the basic form There is no pH that simultaneously has carboxylic acid in the acid form and the amine in the basic form
A. Amino Acids with Electrically Charged Side Chains Negative Pesitive Aspartie Acid Glutamie Acid (Aspi D Lysine Histidine Arginine (Argl R (Glu O (His H Ho Ho NH, -NH, -NH, NH, NH, NH NH H,N ONH, ONH, C. Special Cases B. Amino Acids with Polar Uncharged Side Chains Proline Asparagine (Aani N Cystoine Glycine (Gly © Glutamine Serine Threonine (Pra) P (Ser 9 (The T HO HO Ho -NH, -NH, -NH NH, NH, NH, -NH, но SH он NH, NH, D. Amino Acids with Hydrophobic Side Chains Leucine (Leu O Tryptophan (Trp W Tyrosine Phenylalanine (Phe e Methionine Valine Isoleucine Alanine (Ala A (ValO IMe M Ho Ho Ho HO Ho HO NH, NH, NH, -NH, NH, NH, NH, NH, NH OH If the chromatogram was developed from normal phase conditions, which of the following amino acids is expected to elute first? glycine aspartic acid arginine glutamine
Question 2 Which of the following errors may still produce a chromatogram of amino acids and calculate an Rf value? A) forgot to spray ninhydrin B) solvent level is higher than the spot solvent run over the top of paper C (D) too much sample is applied
d. réčombinants 20. What functional group is found on every amino acid not in a zwitterion form? a. -SH b. -NH, C. -C- d. -S-S- e. no correct response is given 21. The peptide linkage that bonds the monomers together in proteins is also corr
The pKa values for Lysine are 1.92/ 9.99/13.8. At what pH will the amino acid not migrate in an electrical field? Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. a 10.9 b 9.8 c 11.9 d 9.7 e 8.4 f 11.2 g 10.6 h 11.6 i 11.1 im thinking answer is c please clarify
QUESTION 27 In SDS page, the elute from the column first. proteins will move through the gel fastest and first, and in size exclusion chromatography, the proteins will smallest; smallest O smallest, largest O largest; largest largest; smallest QUESTION 28 You have a mixture of positive, negative and neutral proteins. In order to obtain a pure positively charged protein, you can pass this mixture through a column containing O Anion Exchanger beads O G-50 coarse beads O G-50 superfine beads O Cation Exchanger beads