With solution please.... Question 1What type of a relation do we expect to get if we fix the enzyme concentrationand look at velocity as a function of substrate concentration for a single bindingsite enzyme?O linearO sigmoidalO exponentialO hyperbolicQuestion 2Why might we see only one band on the WB when the SDS PAGE demonstratesthat there are multiple bands present?O The primary antibody reacts to the HIS TAG onlyO The other bands do not contain the HIS TAGO The bands missing in the WB have been truncatedO All of the aboveQuestion 3What method below would not be useful to identify a protein?O Mass SpecO Gas ChromatographyO Peptide Mass FingerprintingO Western blotting

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Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question 1 What type of a relation do we expect to get if we fix the enzyme concentration and look at velocity as a function of substrate concentration for a single binding site enzyme? linear sigmoidal exponential O hyperbolic