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Question 1 What type of a relation do we expect to get if we fix the enzyme concentration and look at velocity as a function of substrate concentration for a single binding site enzyme? linear sigmoidal exponential O hyperbolic

Question 1 What type of a relation do we expect to get if we fix the enzyme concentration and look at velocity as a function of substrate concentration for a single binding site enzyme? linear sigmoidal exponential O hyperbolic

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question 1 What type of a relation do we expect to get if we fix the enzyme concentration and look at velocity as a function of substrate concentration for a single binding site enzyme? O linear O sigmoidal O exponential O hyperbolic Question 2 Why might we see only one band on the WB when the SDS PAGE demonstrates that there are multiple bands present? O The primary antibody reacts to the HIS TAG only O The other bands do not contain the HIS TAG O The bands missing in the WB have been truncated O All of the above Question 3 What method below would not be useful to identify a protein? O Mass Spec O Gas Chromatography O Peptide Mass Fingerprinting O Western blotting
Transcribed Image Text:Question 1 What type of a relation do we expect to get if we fix the enzyme concentration and look at velocity as a function of substrate concentration for a single binding site enzyme? O linear O sigmoidal O exponential O hyperbolic Question 2 Why might we see only one band on the WB when the SDS PAGE demonstrates that there are multiple bands present? O The primary antibody reacts to the HIS TAG only O The other bands do not contain the HIS TAG O The bands missing in the WB have been truncated O All of the above Question 3 What method below would not be useful to identify a protein? O Mass Spec O Gas Chromatography O Peptide Mass Fingerprinting O Western blotting
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