PMSF is a molecule used in biochemical research. It inactivates serine proteases by binding covalently to the catalytic serine residue at the active site. Which kind of inhibition does PMSF do? Suicide Mixed Competitive O Non-competitive

The graph displays the activities of wild-type and several mutated forms of subtilisin on a logarithmic scale. 5 The mutations are identified as: • The first letter is the one-letter abbreviation for the amino acid being altered. • The number identifies the position of the residue in the primary structure. The second letter is the one-letter abbreviation for the amino acid replacing the original one. • Uncat. refers to the estimated rate for the uncatalyzed reaction. Log ₁0k cat (S-¹) -5 -10 Wild type D32A S221A H64A İnti. S221A H64A D32A Uncat. How would the activity of a reaction catalyzed by a version of subtilisin with all three residues in the catalytic triad mutated compare to the activity of the uncatalyzed reaction? O It would have more activity, because the reaction catalyzed by the triple mutant is approximately 1000-fold faster than the uncatalyzed reaction. It would have more activity, because the reaction catalyzed by the triple mutant is approximately three-fold faster…

True/Faise Question: Enzymes are usually stereoselective for their substrates (can preferentially bind and act only on a D- or an L-isomer of the substrate). O True O ralse

I once got non specific and another time i got uncompetitive and anothe time i got transition state analog please put the right answer