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- Discuss the uses of PCR and RT-PCR in human identity (e.g. paternity, forensics or criminal cases) as well as in viral or bacterial DNA/RNA analysis (i.e. Identification of SARS-COV-2 RNA coronavirus using RT-PCR).a. DNA fingerprinting is a method of visualizing similarities and differences in the DNA sequence of individuals. Arrange the following items so that they show the DNA fingerprinting steps in proper order: - Gel electrophoresis - Stain or probe for DNA - cut DNA into fragments - Amplify DNA sample using PCR b. On the gel shown below are four DNA samples. Samples A to C are taken from tissues of landslide victims that are being identified, while sample D came from a hair sample brought by a mother looking for the remains of her son. (see img) i. If similar band patterns in a gel are created using the same restriction enzyme, what does that tell you about the DNA sequence of the samples? ii. In sample C, only two fragments were created. How many restriction sites (regions where enzymes cut) are present in sample C?Explain how and why PCR can be used to amplify DNA. Describe the steps in the process. Be sure to address the role of Taq DNA polymerase.
- Explain the concepts of excitation and emission in fluorescence in qRT-PCRComprehensively discuss the principle and applications of PCR. Discuss the quality criteria for nucleic acid isolates. Explain the use of UV spectrometry in nucleic acid purification.Briefly describe the functions of the components of a typical PCR reaction.
- Answer the following: What is DNA fingerprinting? Name 2 applications of DNA fingerprinting from lecture. • Describe the steps of DNA fingerprinting. Include the following terms underlined: o DNA sample o PCR o restriction enzyme o gel electrophoresis If you haven't done so already, explain the steps of PCR in detail - also explain the steps of gel electrophoresis in detail.What is DNA fingerprinting? Mention its application.RECOMBINANT DNA BRIEFLY, DESCRIBE RECOMBINANT DNA AND GIVE ONE CONCRETE EXAMPLE. EVALUATE THE SIGNIFICANCE/PRACTICAL APPLICATIONS OF THIS DNA TECHNOLOGY BY CONSIDERING ETHICAL AND MORAL IMPLICATIONS BEHIND IT. RECOMBINANT DNA EXAMPLE: MODIFIED TRAIT GENE MODIFICATION RECIPIENT ORGANISM FIELDS OF APPLICATION ALSO, WHAT ARE YOUR INSIGHTS? IN 2 TO 3 SENTENCES.