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What are two of the four types of tetrads that come from yeast sexual reproduction?
Explain how the radioactive virus (32P vs35S) tests that show DNA is important. (hint: talk about what DNA is made of...it isn't sulfur!)
Describe one way an SRY mutation can cause differences in sexual
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- Map distances were determined for four differentgenes (MAT, HIS4, THR4, and LEU2) on chromosome III of the yeast Saccharomyces cerevisiae:HIS4 ↔ MAT 37 cMTHR4 ↔ LEU2 35 cMLEU2 ↔ HIS4 23 cMMAT ↔ LEU2 16 cMMAT ↔ THR4 20 cMWhat is the order of genes on the chromosome?Let's say that an experiment resulted in the production of several unattached kinetochores. What effect, if any, would this have on (i) the duration (length) of the mitotic phase, (ii) the timing of cyclin B’s appearance or disappearance, and (iii) the period of time for which MPF is active? Briefly explain your reasoning.One reason that worker bees forgo their own reproduction to help their sister (the queen) reproduce is that female bees are more closely related to their sisters than they are to their own offspring. This quirk of genetics results from the fact that bees have haplodiploid sex determination, in which females are diploid, with a mother and a father, but males are haploid, developing from unfertilized eggs. Because males are haploid, they produce sperm by mitosis. Explainwhy haplodiploid sex determination causes females to be more closely related to their sisters than to their offspring.
- If you expose a culture of human cells (for example,HeLa cells) to 3H-thymidine during S phase, howwould the radioactivity be distributed over a pair ofhomologous chromosomes at metaphase? Would theradioactivity be in (a) one chromatid of one homolog,(b) both chromatids of one homolog, (c) one chromatid each of both homologs, (d) both chromatids ofboth homologs, or (e) some other pattern? Choose thecorrect answer and explain your reasoning.A typical somatic cell from a badger, which is a diploid, sexually reproducing animal, contains a total of 32 chromosomes. Gametogenesis in badgers is similar to humans. What is the genome size for the badger in terms of chromosome number? How many DNA molecules would be found in a secondary oocyte before dividing? How many chromosomes would be found in a cell at metaphase? How many DNA molecules should be in a tetraploid liver cell from the badger?The Yellow Fever vector mosquito (Aedes Aegypti) is normally 2n = 8. Please draw the following diagrams and in each of your diagrams label the following (or note the absence of): a) a pair of homologous chromosomes b) a pair of sister chromatids A) A somatic cell in this organism undergoing prophase. B) A germ cell undergoing anaphase II.
- In an electrophoretic gel across which is applied a powerful electrical alternating pulsed field, the DNA of the haploid fungus Neurospora crassa (n = 7) moves slowly but eventually forms seven bands, which represent DNA fractions that are of different sizes and hence have moved at different speeds. These bands are presumed to be the seven chromosomes. How would you show which band corresponds to which chromosome?Humans and many other complex organisms are diploid, possessing two sets of genes, one inherited from the mother and one from the father. However, a number of eukaryotic organisms spend most of their life cycles in a haploid state. Many of these eukaryotes, such as Neurospora and yeast, still undergo meiosis and sexual reproduction, but most of the cells that make up the organism are haploid. Considering that haploid organisms are fully capable of sexual reproduction and generating genetic variation, why are most complex eukaryotes diploid? In other words, what might be the evolutionary advantage of existing in a diploid state instead of a haploid state? And why might a few organisms, such as Neurospora and yeast, exist as haploids?Consider a species of wasp in which females are diploid and come from fertilized eggs, while males are haploid and come from unfertilized eggs. Assuming that a queen mates with only one male, determine the coefficient of relationship (r) for each of the following. a)A female (worker) to her sister: b)A female (worker) to her brother: c)A female (worker) to her father
- Why is parthenogenesis in bees considered to be a form of asexual reproduction? Select one: a. The male drone is made from a haploid egg. Fertilization is not part of the process to make a male drone. b. The formation of the original haploid cell was achieved using binary fission. c. Although genetic material is transferred from one cell to another using conjugation, the resulting cells will be identical to each other. d. The haploid male bees were made through the process of meiosis, so they are identical to the parent egg cell.In yeast, LYS5, ADE1, and URA2 are genes required to synthesize lysine, adenine and uracil, respectively. A MATa lys5 ADE1 URA2 haploid was mated to a MATA LYS5 ade1 ura2 haploid to make a triply heterozygous diploid. This diploid was put through meiosis, 100 tetrads were dissected and the spore colonies transferred to medium lacking either lysine, adenine or uracil to determine which spores were prototrophic or auxotrophic for those nutrients. The following results were obtained: LYS5-ADE1 ADE1-URA2 LYS5-URA2 PD NPD Ι 20 22 58 18 20 62 50 8 42 Based on this information, select the statements below that are TRUE. Select 4 correct answer(s) A) The LYS5 and URA2 genes are linked. B) The genotypes of the four spores in the ADE1-URA2 tetratypes is ade1 ura2 ade1 URA2 ADE1 ura2 ADE1 URA2 C) The map distance between ADE1 and URA2 is 51 CM. D) ADE1 and URA2 are likely located on the same chromosome. E) The map distance between LYS5 and URA2 is 29 CM. ☐ F) LYS5 and ADE1 independently assort…Let's say that an experiment had no disruption to kinetochores and you are able to inactivate the CDC 14 phosphatase, which acts to remove phosphate groups from substrates that were previously phosphorylated by active MPF. What effect, if any, would this have on (i) the duration (length) of the mitotic phase, (ii) the timing of cyclin B’s appearance or disappearance, and (iii) the period of time for which MPF is active? Briefly explain your reasoning